8PNB

HRV empty capsid

これはPDB形式変換不可エントリーです。

8PNB の概要

• エントリーDOI: 10.2210/pdb8pnb/pdb
• EMDBエントリー: 17780
• 分子名称: Capsid protein VP1, Capsid protein VP2, Genome polyprotein (3 entities in total)
• 機能のキーワード: cryo-em, hrv-b14, ssrna virus, capsid, virus
• 由来する生物種: rhinovirus B14; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 80373.92

構造登録者

Gil-Cantero, D.,Mata, C.P.,Mateu, M.G.,Caston, J.R. (登録日: 2023-06-30, 公開日: 2024-11-20, 最終更新日: 2025-07-02)

主引用文献

Gil-Cantero, D.,Mata, C.P.,Valiente, L.,Rodriguez-Huete, A.,Valbuena, A.,Twarock, R.,Stockley, P.G.,Mateu, M.G.,Caston, J.R.
Cryo-EM of human rhinovirus reveals capsid-RNA duplex interactions that provide insights into virus assembly and genome uncoating.
Commun Biol, 7:1501-1501, 2024

PubMed Abstract: The cryo-EM structure of the human rhinovirus B14 determined in this study reveals 13-bp RNA duplexes symmetrically bound to regions around each of the 30 two-fold axes in the icosahedral viral capsid. The RNA duplexes (~12% of the ssRNA genome) define a quasi-dodecahedral cage that line a substantial part of the capsid interior surface. The RNA duplexes establish a complex network of non-covalent interactions with pockets in the capsid inner wall, including coulombic interactions with a cluster of basic amino acid residues that surround each RNA duplex. A direct comparison was made between the cryo-EM structure of RNA-filled virions and that of RNA-free (empty) capsids that resulted from genome release from a small fraction of viruses. The comparison reveals that some specific residues involved in capsid-duplex RNA interactions in the virion undergo remarkable conformational rearrangements upon RNA release from the capsid. RNA release is also associated with the asynchronous opening of channels at the 30 two-fold axes. The results provide further insights into the molecular mechanisms leading to assembly of rhinovirus particles and their genome uncoating during infection. They may also contribute to development of novel antiviral strategies aimed at interfering with viral capsid-genome interactions during the infectious cycle.

PubMed: 39537894
DOI: 10.1038/s42003-024-07213-2

実験手法

ELECTRON MICROSCOPY (3.8 Å)