8PMM

Structure of Nal1 protein, allele SPIKE from japonica rice, construct 31-458

This is a non-PDB format compatible entry.

Summary for 8PMM

• Entry DOI: 10.2210/pdb8pmm/pdb
• Descriptor: Protein NARROW LEAF 1, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: serine protease, plant protein
• Biological source: Oryza sativa Japonica Group (Japanese rice)
• Total number of polymer chains: 3
• Total formula weight: 143500.57

Authors

Huang, L.Y.,Rety, S.,Xi, X.G. (deposition date: 2023-06-29, release date: 2024-04-17, Last modification date: 2024-11-06)

Primary citation

Huang, L.Y.,Liu, N.N.,Chen, W.F.,Ai, X.,Li, H.H.,Zhang, Z.L.,Hou, X.M.,Fosse, P.,Mauffret, O.,Lei, D.S.,Rety, S.,Xi, X.G.
The catalytic triad of rice NARROW LEAF1 involves H234.
Nat.Plants, 10:743-748, 2024

PubMed Abstract: NARROW LEAF1 (NAL1) exerts a multifaceted influence on leaf morphology and crop yield. Recent crystal study proposed that histidine 233 (H233) is part of the catalytic triad. Here we report that unlike suggested previously, H234 instead of H233 is a component of the catalytic triad alongside residues D291 and S385 in NAL1. Remarkably, residue 233 unexpectedly plays a pivotal role in regulating NAL1's proteolytic activity. These findings establish a strong foundation for utilizing NAL1 in breeding programs aimed at improving crop yield.

PubMed: 38600265
DOI: 10.1038/s41477-024-01668-1

Experimental method

X-RAY DIFFRACTION (1.75 Å)