8PJC

Crystal structure of human insulin desB30 precursor with an Alanine-Alanine-Lysine C-peptide in dimer (T2) conformation

8PJC の概要

• エントリーDOI: 10.2210/pdb8pjc/pdb
• 分子名称: Insulin (2 entities in total)
• 機能のキーワード: insulin, precursor, dimer, hormone
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5969.87

構造登録者

Schluckebier, G.,Johansson, E. (登録日: 2023-06-23, 公開日: 2023-11-08, 最終更新日: 2024-10-16)

主引用文献

Kjeldsen, T.,Andersen, A.S.,Hubalek, F.,Johansson, E.,Kreiner, F.F.,Schluckebier, G.,Kurtzhals, P.
Molecular engineering of insulin for recombinant expression in yeast.
Trends Biotechnol, 42:464-478, 2024

PubMed Abstract: Since the first administration of insulin to a person with diabetes in 1922, scientific contributions from academia and industry have improved insulin therapy and access. The pharmaceutical need for insulin is now more than 40 tons annually, half of which is produced by recombinant secretory expression in Saccharomyces cerevisiae. We discuss how, in this yeast species, adaptation of insulin precursors by removable structural elements is pivotal for efficient secretory expression. The technologies reviewed have been implemented at industrial scale and are seminal for the supply of human insulin and insulin analogues to people with diabetes now and in the future. Engineering of a target protein with removable structural elements may provide a general approach to yield optimisation.

PubMed: 37880066
DOI: 10.1016/j.tibtech.2023.09.012

実験手法

X-RAY DIFFRACTION (2.14 Å)