8PHB

Crystal structure of apo Cami1

8PHB の概要

• エントリーDOI: 10.2210/pdb8phb/pdb
• 分子名称: CRISPR-associated protein, APE2256 family (2 entities in total)
• 機能のキーワード: crispr, cyclic oligoadenylate, rnase, rele-toxin, hydrolase
• 由来する生物種: Allochromatium vinosum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91808.23

構造登録者

Tamulaitiene, G.,Tamulaitis, G.,Mogila, I.,Keda, K. (登録日: 2023-06-19, 公開日: 2023-12-13, 最終更新日: 2024-03-27)

主引用文献

Mogila, I.,Tamulaitiene, G.,Keda, K.,Timinskas, A.,Ruksenaite, A.,Sasnauskas, G.,Venclovas, C.,Siksnys, V.,Tamulaitis, G.
Ribosomal stalk-captured CARF-RelE ribonuclease inhibits translation following CRISPR signaling.
Science, 382:1036-1041, 2023

PubMed Abstract: Prokaryotic type III CRISPR-Cas antiviral systems employ cyclic oligoadenylate (cA) signaling to activate a diverse range of auxiliary proteins that reinforce the CRISPR-Cas defense. Here we characterize a class of cA-dependent effector proteins named CRISPR-Cas-associated messenger RNA (mRNA) interferase 1 (Cami1) consisting of a CRISPR-associated Rossmann fold sensor domain fused to winged helix-turn-helix and a RelE-family mRNA interferase domain. Upon activation by cyclic tetra-adenylate (cA), Cami1 cleaves mRNA exposed at the ribosomal A-site thereby depleting mRNA and leading to cell growth arrest. The structures of apo-Cami1 and the ribosome-bound Cami1-cA complex delineate the conformational changes that lead to Cami1 activation and the mechanism of Cami1 binding to a bacterial ribosome, revealing unexpected parallels with eukaryotic ribosome-inactivating proteins.

PubMed: 38033086
DOI: 10.1126/science.adj2107

実験手法

X-RAY DIFFRACTION (1.7 Å)