8PHA

O(S)-methyltransferase from Pleurotus sapidus

8PHA の概要

• エントリーDOI: 10.2210/pdb8pha/pdb
• 分子名称: O-methyltransferase domain-containing protein, GLYCEROL, 2-HYDROXY BUTANE-1,4-DIOL, ... (6 entities in total)
• 機能のキーワード: biocatalysis, enzymes, fungi, o-methyltransferase, pleurotus sapidus, s-methyltransferase, transferase
• 由来する生物種: Pleurotus sapidus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 215689.43

構造登録者

Korf, L.,Essen, L.-O. (登録日: 2023-06-19, 公開日: 2024-03-27, 最終更新日: 2025-12-24)

主引用文献

Brescia, F.F.,Korf, L.,Essen, L.O.,Zorn, H.,Ruehl, M.
A Novel O - and S -Methyltransferase from Pleurotus sapidus Is Involved in Flavor Formation.
J.Agric.Food Chem., 72:6471-6480, 2024

PubMed Abstract: Increasing consumer aversion to non-natural flavoring substances is prompting a heightened interest in enzymatic processes for flavor production. This includes methylation reactions, which are often performed by using hazardous chemicals. By correlation of aroma profile data and transcriptomic analysis, a novel -methyltransferase (OMT) catalyzing a respective reaction within the formation of -anisaldehyde was identified in the mushroom . Heterologous expression in followed by purification allowed for further characterization of the enzyme. Besides -hydroxybenzaldehyde, the proposed precursor of -anisaldehyde, the enzyme catalyzed the methylation of further hydroxylated aromatic compounds at the - and -position. The values determined for -hydroxybenzaldehyde and -adenosyl-l-methionine were 80 and 107 μM, respectively. Surprisingly, the studied enzyme enabled the transmethylation of thiol-nucleophiles, as indicated by the formation of 2-methyl-3-(methylthio)furan from 2-methyl-3-furanthiol. Moreover, the enzyme was crystallized at a resolution of 2.0 Å, representing the first published crystal structure of a basidiomycetous OMT.

PubMed: 38462720
DOI: 10.1021/acs.jafc.3c08849

実験手法

X-RAY DIFFRACTION (2.02 Å)