8PFR

Mouse RPL39L integrated into the yeast 60S ribosomal subunit

これはPDB形式変換不可エントリーです。

8PFR の概要

• エントリーDOI: 10.2210/pdb8pfr/pdb
• EMDBエントリー: 17653
• 分子名称: 25S rRNA, 60S ribosomal protein L33-A, 60S ribosomal protein L9-A, ... (49 entities in total)
• 機能のキーワード: rpl39, rpl39l, ribosomal protein, protein exit tunnel, ribosome
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 43
• 化学式量合計: 1933033.33

構造登録者

Rabl, J.,Banerjee, A.,Boehringer, D.,Zavolan, M. (登録日: 2023-06-16, 公開日: 2024-06-26, 最終更新日: 2024-12-25)

主引用文献

Banerjee, A.,Ataman, M.,Smialek, M.J.,Mookherjee, D.,Rabl, J.,Mironov, A.,Mues, L.,Enkler, L.,Coto-Llerena, M.,Schmidt, A.,Boehringer, D.,Piscuoglio, S.,Spang, A.,Mittal, N.,Zavolan, M.
Ribosomal protein RPL39L is an efficiency factor in the cotranslational folding of a subset of proteins with alpha helical domains.
Nucleic Acids Res., 52:9028-9048, 2024

PubMed Abstract: Increasingly many studies reveal how ribosome composition can be tuned to optimally translate the transcriptome of individual cell types. In this study, we investigated the expression pattern, structure within the ribosome and effect on protein synthesis of the ribosomal protein paralog 39L (RPL39L). With a novel mass spectrometric approach we revealed the expression of RPL39L protein beyond mouse germ cells, in human pluripotent cells, cancer cell lines and tissue samples. We generated RPL39L knock-out mouse embryonic stem cell (mESC) lines and demonstrated that RPL39L impacts the dynamics of translation, to support the pluripotency and differentiation, spontaneous and along the germ cell lineage. Most differences in protein abundance between WT and RPL39L KO lines were explained by widespread autophagy. By CryoEM analysis of purified RPL39 and RPL39L-containing ribosomes we found that, unlike RPL39, RPL39L has two distinct conformations in the exposed segment of the nascent peptide exit tunnel, creating a distinct hydrophobic patch that has been predicted to support the efficient co-translational folding of alpha helices. Our study shows that ribosomal protein paralogs provide switchable modular components that can tune translation to the protein production needs of individual cell types.

PubMed: 39041433
DOI: 10.1093/nar/gkae630

実験手法

ELECTRON MICROSCOPY (2.15 Å)