8PEJ

CjGH35 with a Galactosidase Activity-Based Probe

8PEJ の概要

• エントリーDOI: 10.2210/pdb8pej/pdb
• 分子名称: Beta-galactosidase, putative, bgl35A, SODIUM ION, (1~{S},2~{S},4~{S},5~{R})-6-(hydroxymethyl)cyclohexane-1,2,3,4,5-pentol, ... (5 entities in total)
• 機能のキーワード: beta-galactosidase, complex, activity-based probe, hydrolase
• 由来する生物種: Cellvibrio japonicus Ueda107
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 500200.91

構造登録者

Offen, W.A.,Davies, G.J. (登録日: 2023-06-14, 公開日: 2023-10-04, 最終更新日: 2024-10-23)

主引用文献

Kuo, C.L.,Su, Q.,van den Nieuwendijk, A.M.C.H.,Beenakker, T.J.M.,Offen, W.A.,Willems, L.I.,Boot, R.G.,Sarris, A.J.,Marques, A.R.A.,Codee, J.D.C.,van der Marel, G.A.,Florea, B.I.,Davies, G.J.,Overkleeft, H.S.,Aerts, J.M.F.G.
The development of a broad-spectrum retaining beta-exo-galactosidase activity-based probe.
Org.Biomol.Chem., 21:7813-7820, 2023

PubMed Abstract: Acid β-galactosidase (GLB1) and galactocerebrosidase (GALC) are retaining exo-β-galactosidases involved in lysosomal glycoconjugate metabolism. Deficiency of GLB1 may result in the lysosomal storage disorders GM1 gangliosidosis, Morquio B syndrome, and galactosialidosis, and deficiency of GALC may result in Krabbe disease. Activity-based protein profiling (ABPP) is a powerful technique to assess the activity of retaining glycosidases in relation to health and disease. This work describes the use of fluorescent and biotin-carrying activity-based probes (ABPs) to assess the activity of both GLB1 and GALC in cell lysates, culture media, and tissue extracts. The reported ABPs, which complement the growing list of retaining glycosidase ABPs based on configurational isomers of cyclophellitol, should assist in fundamental and clinical research on various β-galactosidases, whose inherited deficiencies cause debilitating lysosomal storage disorders.

PubMed: 37724332
DOI: 10.1039/d3ob01261a

実験手法

X-RAY DIFFRACTION (1.5 Å)