8PEH

Crystal structure of Lotus japonicus SYMRK kinase domain D738N

8PEH の概要

• エントリーDOI: 10.2210/pdb8peh/pdb
• 分子名称: Receptor-like kinase SYMRK, SULFATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: kinase, symbiosis, plant, phosphorylation, plant protein
• 由来する生物種: Lotus japonicus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 102826.49

構造登録者

Noergaard, M.M.M.,Gysel, K.,Hansen, S.B.,Andersen, K.R. (登録日: 2023-06-14, 公開日: 2024-02-28, 最終更新日: 2024-11-13)

主引用文献

Abel, N.B.,Norgaard, M.M.M.,Hansen, S.B.,Gysel, K.,Diez, I.A.,Jensen, O.N.,Stougaard, J.,Andersen, K.R.
Phosphorylation of the alpha-I motif in SYMRK drives root nodule organogenesis.
Proc.Natl.Acad.Sci.USA, 121:e2311522121-e2311522121, 2024

PubMed Abstract: Symbiosis receptor-like kinase SYMRK is required for root nodule symbiosis between legume plants and nitrogen-fixing bacteria. To understand symbiotic signaling from SYMRK, we determined the crystal structure to 1.95 Å and mapped the phosphorylation sites onto the intracellular domain. We identified four serine residues in a conserved "alpha-I" motif, located on the border between the kinase core domain and the flexible C-terminal tail, that, when phosphorylated, drives organogenesis. Substituting the four serines with alanines abolished symbiotic signaling, while substituting them with phosphorylation-mimicking aspartates induced the formation of spontaneous nodules in the absence of bacteria. These findings show that the signaling pathway controlling root nodule organogenesis is mediated by SYMRK phosphorylation, which may help when engineering this trait into non-legume plants.

PubMed: 38363863
DOI: 10.1073/pnas.2311522121

実験手法

X-RAY DIFFRACTION (1.95 Å)