8PD8
cAMP-bound SpSLC9C1 in lipid nanodiscs, dimer
Summary for 8PD8
| Entry DOI | 10.2210/pdb8pd8/pdb |
| EMDB information | 17605 |
| Descriptor | Sperm-specific sodium proton exchanger, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE (2 entities in total) |
| Functional Keywords | slc9, nhe, sperm-specific, membrane protein |
| Biological source | Strongylocentrotus purpuratus (purple sea urchin) |
| Total number of polymer chains | 2 |
| Total formula weight | 295907.38 |
| Authors | Kalienkova, V.,Peter, M.,Rheinberger, J.,Paulino, C. (deposition date: 2023-06-12, release date: 2023-11-08, Last modification date: 2023-11-15) |
| Primary citation | Kalienkova, V.,Peter, M.F.,Rheinberger, J.,Paulino, C. Structures of a sperm-specific solute carrier gated by voltage and cAMP. Nature, 623:202-209, 2023 Cited by PubMed Abstract: The newly characterized sperm-specific Na/H exchanger stands out by its unique tripartite domain composition. It unites a classical solute carrier unit with regulatory domains usually found in ion channels, namely, a voltage-sensing domain and a cyclic-nucleotide binding domain, which makes it a mechanistic chimera and a secondary-active transporter activated strictly by membrane voltage. Our structures of the sea urchin SpSLC9C1 in the absence and presence of ligands reveal the overall domain arrangement and new structural coupling elements. They allow us to propose a gating model, where movements in the voltage sensor indirectly cause the release of the exchanging unit from a locked state through long-distance allosteric effects transmitted by the newly characterized coupling helices. We further propose that modulation by its ligand cyclic AMP occurs by means of disruption of the cytosolic dimer interface, which lowers the energy barrier for S4 movements in the voltage-sensing domain. As SLC9C1 members have been shown to be essential for male fertility, including in mammals, our structure represents a potential new platform for the development of new on-demand contraceptives. PubMed: 37880361DOI: 10.1038/s41586-023-06629-w PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.3 Å) |
Structure validation
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