8P61
Crystal structure of O'nyong'nyong virus capsid protease (106-256)
Summary for 8P61
| Entry DOI | 10.2210/pdb8p61/pdb |
| Descriptor | Capsid protein, GLYCEROL, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | capsid protease, onyongnyong virus, alphaviruses, virus |
| Biological source | O'nyong-nyong virus strain Gulu |
| Total number of polymer chains | 4 |
| Total formula weight | 67992.77 |
| Authors | Plewka, J.,Chykunova, Y.,Wilk, P.,Sienczyk, M.,Dubin, G.,Pyrc, K. (deposition date: 2023-05-24, release date: 2024-03-06, Last modification date: 2024-05-15) |
| Primary citation | Chykunova, Y.,Plewka, J.,Wilk, P.,Sienczyk, M.,Dubin, G.,Pyrc, K. Autoinhibition of suicidal capsid protease from O'nyong'nyong virus. Int.J.Biol.Macromol., 262:130136-130136, 2024 Cited by PubMed Abstract: Alphaviruses pose a significant threat to public health. Capsid protein encoded in the alphaviral genomes constitutes an interesting therapy target, as it also serves as a protease (CP). Remarkably, it undergoes autoproteolysis, leading to the generation of the C-terminal tryptophan that localizes to the active pocket, deactivating the enzyme. Lack of activity hampers the viral replication cycle, as the virus is not capable of producing the infectious progeny. We investigated the structure and function of the CP encoded in the genome of O'nyong'nyong virus (ONNV), which has instigated outbreaks in Africa. Our research provides a high-resolution crystal structure of the ONNV CP in its active state and evaluates the enzyme's activity. Furthermore, we demonstrated a dose-dependent reduction in ONNV CP proteolytic activity when exposed to indole, suggesting that tryptophan analogs may be a promising basis for developing small molecule inhibitors. It's noteworthy that the capsid protease plays an essential role in virus assembly, binding viral glycoproteins through its glycoprotein-binding hydrophobic pocket. We showed that non-aromatic cyclic compounds like dioxane disrupt this vital interaction. Our findings provide deeper insights into ONNV's biology, and we believe they will prove instrumental in guiding the development of antiviral strategies against arthritogenic alphaviruses. PubMed: 38354926DOI: 10.1016/j.ijbiomac.2024.130136 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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