8P5O

Proline activating adenylation domain of gramicidin S synthetase 2 - GrsB1-Acore

8P5O の概要

• エントリーDOI: 10.2210/pdb8p5o/pdb
• 分子名称: Gramicidin S synthase 2 (2 entities in total)
• 機能のキーワード: nonribosomal peptide synthetase, adenylation domain, biosynthetic protein
• 由来する生物種: Aneurinibacillus migulanus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 187752.84

構造登録者

Stephan, P.,Basquin, J.,Caputi, L.,O'Connor, S.E.,Kries, H. (登録日: 2023-05-24, 公開日: 2023-07-05, 最終更新日: 2024-10-16)

主引用文献

Stephan, P.,Langley, C.,Winkler, D.,Basquin, J.,Caputi, L.,O'Connor, S.E.,Kries, H.
Directed Evolution of Piperazic Acid Incorporation by a Nonribosomal Peptide Synthetase.
Angew.Chem.Int.Ed.Engl., 62:e202304843-e202304843, 2023

PubMed Abstract: Engineering of biosynthetic enzymes is increasingly employed to synthesize structural analogues of antibiotics. Of special interest are nonribosomal peptide synthetases (NRPSs) responsible for the production of important antimicrobial peptides. Here, directed evolution of an adenylation domain of a Pro-specific NRPS module completely switched substrate specificity to the non-standard amino acid piperazic acid (Piz) bearing a labile N-N bond. This success was achieved by UPLC-MS/MS-based screening of small, rationally designed mutant libraries and can presumably be replicated with a larger number of substrates and NRPS modules. The evolved NRPS produces a Piz-derived gramicidin S analogue. Thus, we give new impetus to the too-early dismissed idea that widely accessible low-throughput methods can switch the specificity of NRPSs in a biosynthetically useful fashion.

PubMed: 37326625
DOI: 10.1002/anie.202304843

実験手法

X-RAY DIFFRACTION (2.6 Å)