8P5E
S. cerevisiae nexus-sCMGE after DNA replication initiation
This is a non-PDB format compatible entry.
Summary for 8P5E
| Entry DOI | 10.2210/pdb8p5e/pdb |
| EMDB information | 17449 |
| Descriptor | DNA replication licensing factor MCM2, DNA replication complex GINS protein SLD5, Cell division control protein 45, ... (19 entities in total) |
| Functional Keywords | saccharomyces cerevisiae, helicase, cmge, initiation of dna replication, dna, dna unwinding, replication |
| Biological source | Saccharomyces cerevisiae (brewer's yeast) More |
| Total number of polymer chains | 15 |
| Total formula weight | 1140442.35 |
| Authors | Henrikus, S.S.,Willhoft, O. (deposition date: 2023-05-24, release date: 2024-05-29, Last modification date: 2024-08-28) |
| Primary citation | Henrikus, S.S.,Gross, M.H.,Willhoft, O.,Puhringer, T.,Lewis, J.S.,McClure, A.W.,Greiwe, J.F.,Palm, G.,Nans, A.,Diffley, J.F.X.,Costa, A. Unwinding of a eukaryotic origin of replication visualized by cryo-EM. Nat.Struct.Mol.Biol., 31:1265-1276, 2024 Cited by PubMed Abstract: To prevent detrimental chromosome re-replication, DNA loading of a double hexamer of the minichromosome maintenance (MCM) replicative helicase is temporally separated from DNA unwinding. Upon S-phase transition in yeast, DNA unwinding is achieved in two steps: limited opening of the double helix and topological separation of the two DNA strands. First, Cdc45, GINS and Polε engage MCM to assemble a double CMGE with two partially separated hexamers that nucleate DNA melting. In the second step, triggered by Mcm10, two CMGEs separate completely, eject the lagging-strand template and cross paths. To understand Mcm10 during helicase activation, we used biochemical reconstitution with cryogenic electron microscopy. We found that Mcm10 splits the double CMGE by engaging the N-terminal homo-dimerization face of MCM. To eject the lagging strand, DNA unwinding is started from the N-terminal side of MCM while the hexamer channel becomes too narrow to harbor duplex DNA. PubMed: 38760633DOI: 10.1038/s41594-024-01280-z PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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