8P37

Structure a catalytically inactive mutant of the IMP dehydrogenase related protein GUAB3 from Synechocystis PCC 6803

8P37 の概要

• エントリーDOI: 10.2210/pdb8p37/pdb
• 分子名称: IMP dehydrogenase subunit, XANTHOSINE-5'-MONOPHOSPHATE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: complex of the enzyme with cofactor and product, biosynthetic protein
• 由来する生物種: Synechocystis sp. PCC 6803
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41566.44

構造登録者

Hernandez-Gomez, A.,Fernandez-Justel, D.,Buey, R.M. (登録日: 2023-05-17, 公開日: 2024-03-27)

主引用文献

Hernandez-Gomez, A.,Irisarri, I.,Fernandez-Justel, D.,Pelaez, R.,Jimenez, A.,Revuelta, J.L.,Balsera, M.,Buey, R.M.
GuaB3, an overlooked enzyme in cyanobacteria's toolbox that sheds light on IMP dehydrogenase evolution.
Structure, 31:1526-1534.e4, 2023

PubMed Abstract: IMP dehydrogenase and GMP reductase are enzymes from the same protein family with analogous structures and catalytic mechanisms that have gained attention because of their essential roles in nucleotide metabolism and as potential drug targets. This study focusses on GuaB3, a less-explored enzyme within this family. Phylogenetic analysis uncovers GuaB3's independent evolution from other members of the family and it predominantly occurs in Cyanobacteria. Within this group, GuaB3 functions as a unique IMP dehydrogenase, while its counterpart in Actinobacteria has a yet unknown function. Synechocystis sp. PCC6803 GuaB3 structures demonstrate differences in the active site compared to canonical IMP dehydrogenases, despite shared catalytic mechanisms. These findings highlight the essential role of GuaB3 in Cyanobacteria, provide insights into the diversity and evolution of the IMP dehydrogenase protein family, and reveal a distinctive characteristic in nucleotide metabolism, potentially aiding in combating harmful cyanobacterial blooms-a growing concern for humans and wildlife.

PubMed: 37875114
DOI: 10.1016/j.str.2023.09.014

実験手法

X-RAY DIFFRACTION (1.219 Å)