8P20

TarM(Se)_G117R-UDP-4RboP-glucose

これはPDB形式変換不可エントリーです。

8P20 の概要

• エントリーDOI: 10.2210/pdb8p20/pdb
• 分子名称: TarM(Se)_G117R, [(2~{R},3~{S},4~{S})-2-[(2~{S},3~{S},4~{R},5~{R},6~{S})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,4-bis(oxidanyl)-5-[oxidanyl-[(2~{R},3~{S},4~{S})-2,3,4-tris(oxidanyl)-5-[oxidanyl-[(2~{R},3~{S},4~{S})-2,3,4,5-tetrakis(oxidanyl)pentoxy]phosphoryl]oxy-pentoxy]phosphoryl]oxy-pentyl] [(2~{S},3~{R},4~{R})-2,3,4-tris(oxidanyl)-5-phosphonooxy-pentyl] hydrogen phosphate, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: staphylococcus epidermidis, wall teichoic acid glycosyltransferase, gt-b fold, alpha-o-glucose., transferase
• 由来する生物種: Staphylococcus epidermidis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 245679.29

構造登録者

Guo, Y.,Stehle, T. (登録日: 2023-05-14, 公開日: 2023-12-06)

主引用文献

Guo, Y.,Du, X.,Krusche, J.,Beck, C.,Ali, S.,Walter, A.,Winstel, V.,Mayer, C.,Codee, J.D.C.,Peschel, A.,Stehle, T.
Invasive Staphylococcus epidermidis uses a unique processive wall teichoic acid glycosyltransferase to evade immune recognition.
Sci Adv, 9:eadj2641-eadj2641, 2023

PubMed Abstract: expresses glycerol phosphate wall teichoic acid (WTA), but some health care-associated methicillin-resistant (HA-MRSE) clones produce a second, ribitol phosphate (RboP) WTA, resembling that of the aggressive pathogen . RboP-WTA promotes HA-MRSE persistence and virulence in bloodstream infections. We report here that the TarM enzyme of HA-MRSE [TarM(Se)] glycosylates RboP-WTA with glucose, instead of -acetylglucosamine (GlcNAc) by TarM(Sa) in . Replacement of GlcNAc with glucose in RboP-WTA impairs HA-MRSE detection by human immunoglobulin G, which may contribute to the immune-evasion capacities of many invasive . Crystal structures of complexes with uridine diphosphate glucose (UDP-glucose), and with UDP and glycosylated poly(RboP), reveal the binding mode and glycosylation mechanism of this enzyme and explain why TarM(Se) and TarM(Sa) link different sugars to poly(RboP). These structural data provide evidence that TarM(Se) is a processive WTA glycosyltransferase. Our study will support the targeted inhibition of TarM enzymes, and the development of RboP-WTA targeting vaccines and phage therapies.

PubMed: 38000019
DOI: 10.1126/sciadv.adj2641

実験手法

X-RAY DIFFRACTION (2.848 Å)