8P0X

Structure of the human Commander complex Retriever Subcomplex

これはPDB形式変換不可エントリーです。

8P0X の概要

• エントリーDOI: 10.2210/pdb8p0x/pdb
• EMDBエントリー: 17341
• 分子名称: Coiled-coil domain-containing protein 93, Coiled-coil domain-containing protein 22, Vacuolar protein sorting-associated protein 29, ... (5 entities in total)
• 機能のキーワード: alpha solenoid, arrestin fold, phosphoesterase fold, complex, unknown function
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 307457.79

構造登録者

Kumpula, E.P.,Laulumaa, S.,Huiskonen, J.T. (登録日: 2023-05-11, 公開日: 2024-03-20, 最終更新日: 2024-07-03)

主引用文献

Laulumaa, S.,Kumpula, E.P.,Huiskonen, J.T.,Varjosalo, M.
Structure and interactions of the endogenous human Commander complex.
Nat.Struct.Mol.Biol., 31:925-938, 2024

PubMed Abstract: The Commander complex, a 16-protein assembly, plays multiple roles in cell homeostasis, cell cycle and immune response. It consists of copper-metabolism Murr1 domain proteins (COMMD1-10), coiled-coil domain-containing proteins (CCDC22 and CCDC93), DENND10 and the Retriever subcomplex (VPS26C, VPS29 and VPS35L), all expressed ubiquitously in the body and linked to various diseases. Here, we report the structure and key interactions of the endogenous human Commander complex by cryogenic-electron microscopy and mass spectrometry-based proteomics. The complex consists of a stable core of COMMD1-10 and an effector containing DENND10 and Retriever, scaffolded together by CCDC22 and CCDC93. We establish the composition of Commander and reveal major interaction interfaces. These findings clarify its roles in intracellular transport, and uncover a strong association with cilium assembly, and centrosome and centriole functions.

PubMed: 38459129
DOI: 10.1038/s41594-024-01246-1

実験手法

ELECTRON MICROSCOPY (7.5 Å)