8P00

Cryo-EM structure of Rotavirus B NSP2

8P00 の概要

• エントリーDOI: 10.2210/pdb8p00/pdb
• EMDBエントリー: 17323
• 分子名称: Non-structural protein 2 (1 entity in total)
• 機能のキーワード: octamer, rna binding, rotavirus, rna chaperone, viral protein
• 由来する生物種: Human rotavirus B strain CAL-1
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 276605.38

構造登録者

Chamera, S.,Nowotny, M. (登録日: 2023-05-09, 公開日: 2024-02-28, 最終更新日: 2024-05-08)

主引用文献

Chamera, S.,Wycisk, K.,Czarnocki-Cieciura, M.,Nowotny, M.
Cryo-EM structure of rotavirus B NSP2 reveals its unique tertiary architecture.
J.Virol., 98:e0166023-e0166023, 2024

PubMed Abstract: Rotavirus (RV) NSP2 is a multifunctional RNA chaperone that exhibits numerous activities that are essential for replication and viral genome packaging. We performed an analysis that highlighted a distant relationship of NSP2 from rotavirus B (RVB) to proteins from other human RVs. We solved a cryo-electron microscopy structure of RVB NSP2 that shows structural differences with corresponding proteins from other human RVs. Based on the structure, we identified amino acid residues that are involved in RNA interactions. Anisotropy titration experiments showed that these residues are important for nucleic acid binding. We also identified structural motifs that are conserved in all RV species. Collectively, our data complete the structural characterization of rotaviral NSP2 protein and demonstrate its structural diversity among RV species.IMPORTANCERotavirus B (RVB), also known as adult diarrhea rotavirus, has caused epidemics of severe diarrhea in China, India, and Bangladesh. Thousands of people are infected in a single RVB epidemic. However, information on this group of rotaviruses remains limited. As NSP2 is an essential protein in the viral life cycle, including its role in the formation of replication factories, it may be a target for future antiviral strategy against viruses with similar mechanisms.

PubMed: 38421167
DOI: 10.1128/jvi.01660-23

実験手法

ELECTRON MICROSCOPY (3.8 Å)