8OZ4

Populus tremula stable protein 1 with an alternate crystal lattice

8OZ4 の概要

• エントリーDOI: 10.2210/pdb8oz4/pdb
• 分子名称: Stable protein 1 (1 entity in total)
• 機能のキーワード: plant protein, protein engineering
• 由来する生物種: Populus tremula
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 37268.89

構造登録者

Sklyar, J.,Zeibaq, Y.,Bachar, O.,Yehezkeli, O.,Adir, N. (登録日: 2023-05-08, 公開日: 2024-03-27, 最終更新日: 2025-04-23)

主引用文献

Zeibaq, Y.,Bachar, O.,Sklyar, J.,Adir, N.,Yehezkeli, O.
A Bioengineered Stable Protein 1-Hemin Complex with Enhanced Peroxidase-Like Catalytic Properties.
Small Sci, 4:2400025-2400025, 2024

PubMed Abstract: Enzymes have gained their unique efficiency and catalytic activity through billions of years of evolution, perfecting their active site to a desired reaction. Inspired by nature, a novel enzyme-mimicking platform is designed based on stable protein 1 (SP1) to create a nano-compartment that mimics peroxidase activity. The biohybrid reveals enhanced activity over the hemin cofactor alone and improved stability in organic solvents in comparison to native peroxidase. Furthermore, the utilization of the obtained biohybrid in an optical glucose biosensing platform is shown. The biohybrid crystallographic structure is solved, indicating that the SP1 structure is not affected by the hemin coordination. This work opens the path for developing new cofactor binding centers in engineered protein scaffolds for various artificial catalytic processes.

PubMed: 40212758
DOI: 10.1002/smsc.202400025

実験手法

X-RAY DIFFRACTION (3.1 Å)