8OVS

Crystal structure of YeGT glycosyltransferase with bound UDP

これはPDB形式変換不可エントリーです。

8OVS の概要

• エントリーDOI: 10.2210/pdb8ovs/pdb
• 分子名称: YeGT glycosyltransferase, URIDINE-5'-DIPHOSPHATE, ACETATE ION, ... (8 entities in total)
• 機能のキーワード: glycosyltransferase, effector protein, nucleotide binding protein, transferase
• 由来する生物種: Yersinia enterocolitica
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71731.82

構造登録者

Wirth, C.,Hunte, C. (登録日: 2023-04-26, 公開日: 2024-05-22, 最終更新日: 2024-06-05)

主引用文献

Schneider, S.,Wirth, C.,Jank, T.,Hunte, C.,Aktories, K.
Tyrosine-modifying glycosylation by Yersinia effectors.
J.Biol.Chem., 300:107331-107331, 2024

PubMed Abstract: Mono-O-glycosylation of target proteins by bacterial toxins or effector proteins is a well-known mechanism by which bacteria interfere with essential functions of host cells. The respective glycosyltransferases are important virulence factors such as the Clostridioides difficile toxins A and B. Here, we describe two glycosyltransferases of Yersinia species that have a high sequence identity: YeGT from the zoonotic pathogen Yersinia enterocolitica and YkGT from the murine pathogen Yersinia kristensenii. We show that both modify Rho family proteins by attachment of GlcNAc at tyrosine residues (Tyr-34 in RhoA). Notably, the enzymes differed in their target protein specificity. While YeGT modified RhoA, B, and C, YkGT possessed a broader substrate spectrum and glycosylated not only Rho but also Rac and Cdc42 subfamily proteins. Mutagenesis studies indicated that residue 177 is important for this broader target spectrum. We determined the crystal structure of YeGT shortened by 16 residues N terminally (sYeGT) in the ligand-free state and bound to UDP, the product of substrate hydrolysis. The structure assigns sYeGT to the GT-A family. It shares high structural similarity to glycosyltransferase domains from toxins. We also demonstrated that the 16 most N-terminal residues of YeGT and YkGT are important for the mediated translocation into the host cell using the pore-forming protective antigen of anthrax toxin. Mediated introduction into HeLa cells or ectopic expression of YeGT and YkGT caused morphological changes and redistribution of the actin cytoskeleton. The data suggest that YeGT and YkGT are likely bacterial effectors belonging to the family of tyrosine glycosylating bacterial glycosyltransferases.

PubMed: 38703997
DOI: 10.1016/j.jbc.2024.107331

実験手法

X-RAY DIFFRACTION (1.123 Å)