8OT4

seeded Abeta(1-40) amyloid fibril (morphology I)

8OT4 の概要

• エントリーDOI: 10.2210/pdb8ot4/pdb
• EMDBエントリー: 17168
• 分子名称: Amyloid-beta A4 protein (1 entity in total)
• 機能のキーワード: amyloid fibril, amyloid-beta, protein fibril
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 52030.22

構造登録者

Pfeiffer, P.B.,Schmidt, M.,Faendrich, M. (登録日: 2023-04-20, 公開日: 2024-02-07)

主引用文献

Pfeiffer, P.B.,Ugrina, M.,Schwierz, N.,Sigurdson, C.J.,Schmidt, M.,Fandrich, M.
Cryo-EM Analysis of the Effect of Seeding with Brain-derived A beta Amyloid Fibrils.
J.Mol.Biol., 436:168422-168422, 2023

PubMed Abstract: Aβ amyloid fibrils from Alzheimer's brain tissue are polymorphic and structurally different from typical in vitro formed Aβ fibrils. Here, we show that brain-derived (ex vivo) fibril structures can be proliferated by seeding in vitro. The proliferation reaction is only efficient for one of the three abundant ex vivo Aβ fibril morphologies, which consists of two peptide stacks, while the inefficiently proliferated fibril morphologies contain four or six peptide stacks. In addition to the seeded fibril structures, we find that de novo nucleated fibril structures can emerge in seeded samples if the seeding reaction is continued over multiple generations. These data imply a competition between de novo nucleation and seed extension and suggest further that seeding favours the outgrowth of fibril morphologies that contain fewer peptide stacks.

PubMed: 38158175
DOI: 10.1016/j.jmb.2023.168422

実験手法

ELECTRON MICROSCOPY (2.97 Å)