8ON2

NI,FE-CODH -600mV state : 24 h Dioxygen Exposure

8ON2 の概要

• エントリーDOI: 10.2210/pdb8on2/pdb
• 分子名称: Carbon monoxide dehydrogenase 2, IRON/SULFUR CLUSTER, FE2/S2 (INORGANIC) CLUSTER, ... (5 entities in total)
• 機能のキーワード: cluster c, 4fe-4s, cytoplasm, iron, iron-sulfur, membrane, metal-binding, nickel, oxidoreductase
• 由来する生物種: Carboxydothermus hydrogenoformans Z-2901
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 67871.01

構造登録者

Basak, Y.,Jeoung, J.-H.,Dobbek, H. (登録日: 2023-03-31, 公開日: 2023-12-06)

主引用文献

Basak, Y.,Jeoung, J.H.,Domnik, L.,Dobbek, H.
Stepwise O 2 -Induced Rearrangement and Disassembly of the [NiFe 4 (OH)( mu 3 -S) 4 ] Active Site Cluster of CO Dehydrogenase.
Angew.Chem.Int.Ed.Engl., 62:e202305341-e202305341, 2023

PubMed Abstract: Ni,Fe-containing carbon monoxide dehydrogenases (CODHs) catalyze the reversible reduction of carbon dioxide to carbon monoxide. CODHs are found in anaerobic microorganisms and can rapidly lose their activity when exposed to air. What causes the loss of activity is unclear. In this study, we analyzed the time-dependent structural changes induced by the presence of air on the metal centers of CODH-II. We show that inactivation is a multistep process. In a reversible step, the open coordination site on the Ni ion is blocked by a Ni,Fe-bridging μ-sulfido or chlorido ligand. Blocking this open coordination site with a cyanide ligand stabilizes the cluster against O -induced decomposition, indicating that O attacks at the Ni ion. In the subsequent irreversible phase, nickel is lost, the Fe ions rearrange and the sulfido ligands disappear. Our data are consistent with a reversible reductive reactivation mechanism to protect CODHs from transient over-oxidation.

PubMed: 37279092
DOI: 10.1002/anie.202305341

実験手法

X-RAY DIFFRACTION (1.58 Å)