8OM3

Small subunit of yeast mitochondrial ribosome in complex with IF3/Aim23.

これはPDB形式変換不可エントリーです。

8OM3 の概要

• エントリーDOI: 10.2210/pdb8om3/pdb
• EMDBエントリー: 16967 17094 17095 17096 17097
• 分子名称: 37S ribosomal protein MRP51, mitochondrial, 37S ribosomal protein S10, mitochondrial, 37S ribosomal protein S18, mitochondrial, ... (40 entities in total)
• 機能のキーワード: mitochondria, initiation factor 3, pre-initiation complex, ribosome
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1513707.17

構造登録者

Itoh, Y.,Chicherin, I.,Kamenski, P.,Amunts, A. (登録日: 2023-03-31, 公開日: 2024-01-10, 最終更新日: 2024-01-31)

主引用文献

Ast, T.,Itoh, Y.,Sadre, S.,McCoy, J.G.,Namkoong, G.,Wengrod, J.C.,Chicherin, I.,Joshi, P.R.,Kamenski, P.,Suess, D.L.M.,Amunts, A.,Mootha, V.K.
METTL17 is an Fe-S cluster checkpoint for mitochondrial translation.
Mol.Cell, 84:359-, 2024

PubMed Abstract: Friedreich's ataxia (FA) is a debilitating, multisystemic disease caused by the depletion of frataxin (FXN), a mitochondrial iron-sulfur (Fe-S) cluster biogenesis factor. To understand the cellular pathogenesis of FA, we performed quantitative proteomics in FXN-deficient human cells. Nearly every annotated Fe-S cluster-containing protein was depleted, indicating that as a rule, cluster binding confers stability to Fe-S proteins. We also observed depletion of a small mitoribosomal assembly factor METTL17 and evidence of impaired mitochondrial translation. Using comparative sequence analysis, mutagenesis, biochemistry, and cryoelectron microscopy, we show that METTL17 binds to the mitoribosomal small subunit during late assembly and harbors a previously unrecognized [FeS] cluster required for its stability. METTL17 overexpression rescued the mitochondrial translation and bioenergetic defects, but not the cellular growth, of FXN-depleted cells. These findings suggest that METTL17 acts as an Fe-S cluster checkpoint, promoting translation of Fe-S cluster-rich oxidative phosphorylation (OXPHOS) proteins only when Fe-S cofactors are replete.

PubMed: 38199006
DOI: 10.1016/j.molcel.2023.12.016

実験手法

ELECTRON MICROSCOPY (2.87 Å)