8OJJ

Cryo-EM structure of the DnaD-NTD tetramer

8OJJ の概要

• エントリーDOI: 10.2210/pdb8ojj/pdb
• EMDBエントリー: 16914
• 分子名称: DNA replication protein DnaD (1 entity in total)
• 機能のキーワード: replication helicase loading, small cryo-em structure, dna binding protein
• 由来する生物種: Bacillus subtilis subsp. subtilis str. 168
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 110702.86

構造登録者

Winterhalter, C.,Pelliciari, S.,Cronin, N.,Costa, T.R.D.,Murray, H.,Ilangovan, A. (登録日: 2023-03-24, 公開日: 2023-05-17, 最終更新日: 2024-07-24)

主引用文献

Winterhalter, C.,Pelliciari, S.,Stevens, D.,Fenyk, S.,Marchand, E.,Cronin, N.B.,Soultanas, P.,Costa, T.R.D.,Ilangovan, A.,Murray, H.
The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin.
Nucleic Acids Res., 51:4322-4340, 2023

PubMed Abstract: Genome replication is a fundamental biological activity shared by all organisms. Chromosomal replication proceeds bidirectionally from origins, requiring the loading of two helicases, one for each replisome. However, the molecular mechanisms underpinning helicase loading at bacterial chromosome origins (oriC) are unclear. Here we investigated the essential DNA replication initiation protein DnaD in the model organism Bacillus subtilis. A set of DnaD residues required for ssDNA binding was identified, and photo-crosslinking revealed that this ssDNA binding region interacts preferentially with one strand of oriC. Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). Considered with single particle cryo-electron microscopy (cryo-EM) imaging of DnaD, we propose that the location of the DRE within oriC orchestrates strand-specific recruitment of helicase during DNA replication initiation. These findings significantly advance our mechanistic understanding of bidirectional replication from a bacterial chromosome origin.

PubMed: 37093985
DOI: 10.1093/nar/gkad277

実験手法

ELECTRON MICROSCOPY (5.47 Å)