8OF0

Structure of the mammalian Pol II-SPT6-Elongin complex, Structure 1

8OF0 の概要

• エントリーDOI: 10.2210/pdb8of0/pdb
• EMDBエントリー: 16836 16840
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11-a, ... (21 entities in total)
• 機能のキーワード: transcription elongation, elongin, rna polymerase ii, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 890993.47

構造登録者

Chen, Y.,Kokic, G.,Dienemann, C.,Dybkov, O.,Urlaub, H.,Cramer, P. (登録日: 2023-03-13, 公開日: 2023-10-18, 最終更新日: 2023-12-27)

主引用文献

Chen, Y.,Kokic, G.,Dienemann, C.,Dybkov, O.,Urlaub, H.,Cramer, P.
Structure of the transcribing RNA polymerase II-Elongin complex.
Nat.Struct.Mol.Biol., 30:1925-1935, 2023

PubMed Abstract: Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA binds the RPB2 side of Pol II and anchors the ELOB-ELOC subunit heterodimer. ELOA contains a 'latch' that binds between the end of the Pol II bridge helix and funnel helices, thereby inducing a conformational change near the polymerase active center. The latch is required for the elongation-stimulatory activity of Elongin, but not for Pol II binding, indicating that Elongin functions by allosterically regulating the conformational mobility of the polymerase active center. Elongin binding to Pol II is incompatible with association of the super elongation complex, PAF1 complex and RTF1, which also contain an elongation-stimulatory latch element.

PubMed: 37932450
DOI: 10.1038/s41594-023-01138-w

実験手法

ELECTRON MICROSCOPY (3.05 Å)