8KI0

Crystal structure of the hemophore HasA from Pseudomonas protegens Pf-5 capturing Fe-tetraphenylporphyrin

8KI0 の概要

• エントリーDOI: 10.2210/pdb8ki0/pdb
• 分子名称: Heme acquisition protein HasAp, [5,10,15,20-tetraphenylporphyrinato(2-)-kappa~4~N~21~,N~22~,N~23~,N~24~]iron, 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID, ... (6 entities in total)
• 機能のキーワード: heme acquisition protein, transport protein
• 由来する生物種: Pseudomonas protegens Pf-5
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40165.33

構造登録者

Shisaka, Y.,Inaba, H.,Sugimoto, H.,Shoji, O. (登録日: 2023-08-22, 公開日: 2024-03-27, 最終更新日: 2024-05-15)

主引用文献

Inaba, H.,Shisaka, Y.,Ariyasu, S.,Sakakibara, E.,Ueda, G.,Aiba, Y.,Shimizu, N.,Sugimoto, H.,Shoji, O.
Heme-substituted protein assembly bridged by synthetic porphyrin: achieving controlled configuration while maintaining rotational freedom.
Rsc Adv, 14:8829-8836, 2024

PubMed Abstract: The use of biological host-guest interactions, specifically the binding of hemoprotein to heme, has attracted significant research interest in the design of artificial protein assemblies. However, because of the inherent flexibility of the propionic acid group of heme, it is difficult to control the positioning and orientation of the protein unit and to construct well-ordered structures. Herein, we report a heme-substituted protein dimer composed of the native hemoprotein HasA, which accommodates a tetraphenylporphyrin bearing an additional metal coordination site. The specific binding of the tetraphenylporphyrin with an additional metal coordination site that protrudes in a fixed direction confines the configuration of the dimer structure to a defined bent form. The small-angle X-ray scattering profile shows the dimer structure with a bent form and suggests dynamic rotational behavior while keeping its bent-core structure, resembling a bevel gear. This unique dimer structure demonstrates that the design of heme-substituted protein assemblies can be expanded to protein assemblies while maintaining the rotational freedom of the individual protein units.

PubMed: 38495978
DOI: 10.1039/d4ra01042f

実験手法

X-RAY DIFFRACTION (1.45 Å)