8KD0

Crystal structure of SAR11_0769 from 'Candidatus Pelagibacter ubique' HTCC1062 bound to a co-purified ligand, beta-galactopyranose

8KD0 の概要

• エントリーDOI: 10.2210/pdb8kd0/pdb
• 分子名称: Probable binding protein component of ABC sugar transporter, beta-D-galactopyranose (3 entities in total)
• 機能のキーワード: abc transporter, glucose transporter, periplasmic binding protein, solute-binding protein, transport protein
• 由来する生物種: Candidatus Pelagibacter ubique HTCC1062
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45106.18

構造登録者

Clifton, B.E.,Laurino, P. (登録日: 2023-08-08, 公開日: 2024-07-17, 最終更新日: 2024-10-30)

主引用文献

Clifton, B.E.,Alcolombri, U.,Uechi, G.I.,Jackson, C.J.,Laurino, P.
The ultra-high affinity transport proteins of ubiquitous marine bacteria.
Nature, 634:721-728, 2024

PubMed Abstract: SAR11 bacteria are the most abundant microorganisms in the surface ocean and have global biogeochemical importance. To thrive in their competitive oligotrophic environment, these bacteria rely heavily on solute-binding proteins that facilitate uptake of specific substrates via membrane transporters. The functions and properties of these transport proteins are key factors in the assimilation of dissolved organic matter and biogeochemical cycling of nutrients in the ocean, but they have remained largely inaccessible to experimental investigation. Here we performed genome-wide experimental characterization of all solute-binding proteins in a prototypical SAR11 bacterium, revealing specific functions and general trends in their properties that contribute to the success of SAR11 bacteria in oligotrophic environments. We found that the solute-binding proteins of SAR11 bacteria have extremely high binding affinity (dissociation constant >20 pM) and high binding specificity, revealing molecular mechanisms of oligotrophic adaptation. Our functional data have uncovered new carbon sources for SAR11 bacteria and enable accurate biogeographical analysis of SAR11 substrate uptake capabilities throughout the ocean. This study provides a comprehensive view of the substrate uptake capabilities of ubiquitous marine bacteria, providing a necessary foundation for understanding their contribution to assimilation of dissolved organic matter in marine ecosystems.

PubMed: 39261732
DOI: 10.1038/s41586-024-07924-w

実験手法

X-RAY DIFFRACTION (1.675 Å)