8K9A

Cryo-EM structure of DSR2-DSAD1 state 2

8K9A の概要

• エントリーDOI: 10.2210/pdb8k9a/pdb
• EMDBエントリー: 36982
• 分子名称: SIR2-like domain-containing protein, SPbeta prophage-derived uncharacterized protein YotI (2 entities in total)
• 機能のキーワード: cryo-em structure of a protein, antiviral protein
• 由来する生物種: Bacillus subtilis; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 502289.02

構造登録者

Zhang, H.,Li, Z.,Li, X.Z. (登録日: 2023-07-31, 公開日: 2024-05-01, 最終更新日: 2024-05-08)

主引用文献

Yin, H.,Li, X.,Wang, X.,Zhang, C.,Gao, J.,Yu, G.,He, Q.,Yang, J.,Liu, X.,Wei, Y.,Li, Z.,Zhang, H.
Insights into the modulation of bacterial NADase activity by phage proteins.
Nat Commun, 15:2692-2692, 2024

PubMed Abstract: The Silent Information Regulator 2 (SIR2) protein is widely implicated in antiviral response by depleting the cellular metabolite NAD. The defense-associated sirtuin 2 (DSR2) effector, a SIR2 domain-containing protein, protects bacteria from phage infection by depleting NAD, while an anti-DSR2 protein (DSR anti-defense 1, DSAD1) is employed by some phages to evade this host defense. The NADase activity of DSR2 is unleashed by recognizing the phage tail tube protein (TTP). However, the activation and inhibition mechanisms of DSR2 are unclear. Here, we determine the cryo-EM structures of DSR2 in multiple states. DSR2 is arranged as a dimer of dimers, which is facilitated by the tetramerization of SIR2 domains. Moreover, the DSR2 assembly is essential for activating the NADase function. The activator TTP binding would trigger the opening of the catalytic pocket and the decoupling of the N-terminal SIR2 domain from the C-terminal domain (CTD) of DSR2. Importantly, we further show that the activation mechanism is conserved among other SIR2-dependent anti-phage systems. Interestingly, the inhibitor DSAD1 mimics TTP to trap DSR2, thus occupying the TTP-binding pocket and inhibiting the NADase function. Together, our results provide molecular insights into the regulatory mechanism of SIR2-dependent NAD depletion in antiviral immunity.

PubMed: 38538592
DOI: 10.1038/s41467-024-47030-z

実験手法

ELECTRON MICROSCOPY (3.9 Å)