8K80

Crystal structure of Langya Virus attachment (G) glycoprotein

これはPDB形式変換不可エントリーです。

8K80 の概要

• エントリーDOI: 10.2210/pdb8k80/pdb
• 分子名称: Langya Virus attachment glycoprotein (1 entity in total)
• 機能のキーワード: langya virus; glycoprotein, viral protein
• 由来する生物種: Langya virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 144460.11

構造登録者

Li, Y.H.,Huang, X.Y.,Xu, J.J. (登録日: 2023-07-28, 公開日: 2023-09-13, 最終更新日: 2025-12-31)

主引用文献

Li, Y.,Huang, X.,Zai, X.,Mao, C.,Li, R.,Feng, Y.,Zhang, Y.,Zhang, Z.,Zhang, J.,Xu, J.
Antigenic and structural insights into Langya henipavirus attachment glycoprotein.
Virol Sin, 40:769-777, 2025

PubMed Abstract: The invasion of host cells by the henipavirus is facilitated through the interaction between viral attachment (G) and fusion (F) glycoproteins with receptors on the cell surface. Langya henipavirus (LayV) was newly identified in China in 2022. The G proteins of LayV and Mojiang virus (MojV) exhibit high amino acid homology (86%), while they are located in a unique evolutionary clade within the Henipavirus genus. In this study, the crystal structure of the LayV G protein was resolved at a 3.37 Å resolution, revealing a head domain with six β-propeller-like domains distinct from other henipavirus G proteins, such as those of Nipah virus (NiV) and Hendra virus (HeV). Furthermore, the prominent loop in the center cavity of the LayV G protein showed unique structural features. In the ELISA and SPR assays, the LayV G protein was unable to bind to the existing henipavirus-neutralizing antibodies or the ephrin-B2 receptor. Immunogenicity studies in mice demonstrated robust antibody responses elicited by the LayV G protein. These antibodies exhibited strong reactivity against both LayV and MojV G proteins. However, only weak cross-reactivity was observed with other henipaviruses. Moreover, eight monoclonal antibodies targeting the LayV G protein were generated, two of which exhibited broad binding activity across different henipavirus G proteins. These findings underscore the need for tailored vaccines and therapeutics for LayV and related novel henipaviruses.

PubMed: 40907748
DOI: 10.1016/j.virs.2025.08.005

実験手法

X-RAY DIFFRACTION (3.37 Å)