8K6F

LnaB-Actin-PRUb ternary complex

8K6F の概要

• エントリーDOI: 10.2210/pdb8k6f/pdb
• 分子名称: Actin gamma 1, Legionella effector LnaB, Ubiquitin, ... (5 entities in total)
• 機能のキーワード: ampylation, legionella effector, toxin
• 由来する生物種: Legionella; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 183650.83

構造登録者

Chen, T.T.,Ouyang, S.Y. (登録日: 2023-07-25, 公開日: 2025-01-29, 最終更新日: 2025-08-13)

主引用文献

Chen, T.T.,Lu, Q.,Zheng, S.R.,Fu, J.,Chen, J.,Kang, L.,Wu, J.,Luo, J.,Tong, J.,Li, S.,Li, X.,Li, S.,Li, J.,Wang, S.,Feng, Y.,Luo, Z.Q.,Ouyang, S.
Structure and mechanism of an actin-dependent bacterial phosphoryl AMPylase.
Nat.Chem.Biol., 2025

PubMed Abstract: The two effectors LnaB and MavL of Legionella pneumophila coordinate the conversion of phosphoribosyl ubiquitin (PR-Ub) released by reversal of ubiquitination induced by members of the SidE effector family into functional Ub. LnaB acts as an actin-dependent phosphoryl AMPylase that converts PR-Ub into ADP-ribosylated (ADPR)-Ub. Catalysis by LnaB requires the conserved SHE motif present in a large family of bacterial toxins. Here we describe a series of structures of LnaB in complex with the cofactor actin and the substrate PR-Ub and ATP. LnaB harbors both adenylyltransferase and ATPase activities, which reveal an adenylylation mechanism involved in a two-step catalytic process. Actin performs a unique activation mechanism that promotes the recruitment of PR-Ub by LnaB to activate LnaB's ATPase activity through interacting with LnaB and PR-Ub. Mechanisms derived from this series of structures covering the process of LnaB action establish an important biochemical basis for protein AMPylation.

PubMed: 40588486
DOI: 10.1038/s41589-025-01945-w

実験手法

X-RAY DIFFRACTION (3.41 Å)