8K65

Serial femtosecond crystallography structure of CO bound ba3- type cytochrome c oxidase without pump laser irradiation

Summary for 8K65

• Entry DOI: 10.2210/pdb8k65/pdb
• Descriptor: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide 2A, ... (10 entities in total)
• Functional Keywords: membrane proteins, structural dynamics, serial femtosecond crystallography, time-resolved studies, electron transport
• Biological source: Thermus thermophilus HB8; More
• Total number of polymer chains: 3
• Total formula weight: 92995.23

Authors

Safari, C.,Ghosh, S.,Andersson, R.,Johannesson, J.,Donoso, A.V.,Bath, P.,Zoric, D.,Sandelin, E.,Nango, E.,Tanaka, R.,Iwata, S.,Neutze, R.,Branden, G. (deposition date: 2023-07-25, release date: 2023-11-15, Last modification date: 2024-03-20)

Primary citation

Safari, C.,Ghosh, S.,Andersson, R.,Johannesson, J.,Bath, P.,Uwangue, O.,Dahl, P.,Zoric, D.,Sandelin, E.,Vallejos, A.,Nango, E.,Tanaka, R.,Bosman, R.,Borjesson, P.,Dunevall, E.,Hammarin, G.,Ortolani, G.,Panman, M.,Tanaka, T.,Yamashita, A.,Arima, T.,Sugahara, M.,Suzuki, M.,Masuda, T.,Takeda, H.,Yamagiwa, R.,Oda, K.,Fukuda, M.,Tosha, T.,Naitow, H.,Owada, S.,Tono, K.,Nureki, O.,Iwata, S.,Neutze, R.,Branden, G.
Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome c oxidase.
Sci Adv, 9:eadh4179-eadh4179, 2023

PubMed Abstract: Cytochrome oxidase (CO) is part of the respiratory chain and contributes to the electrochemical membrane gradient in mitochondria as well as in many bacteria, as it uses the energy released in the reduction of oxygen to pump protons across an energy-transducing biological membrane. Here, we use time-resolved serial femtosecond crystallography to study the structural response of the active site upon flash photolysis of carbon monoxide (CO) from the reduced heme of -type CO. In contrast with the -type enzyme, our data show how CO is stabilized on Cu through interactions with a transiently ordered water molecule. These results offer a structural explanation for the extended lifetime of the Cu-CO complex in -type CO and, by extension, the extremely high oxygen affinity of the enzyme.

PubMed: 38064560
DOI: 10.1126/sciadv.adh4179

Experimental method

X-RAY DIFFRACTION (2 Å)