8K65

Serial femtosecond crystallography structure of CO bound ba3- type cytochrome c oxidase without pump laser irradiation

8K65 の概要

• エントリーDOI: 10.2210/pdb8k65/pdb
• 分子名称: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide 2A, ... (10 entities in total)
• 機能のキーワード: membrane proteins, structural dynamics, serial femtosecond crystallography, time-resolved studies, electron transport
• 由来する生物種: Thermus thermophilus HB8; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 92995.23

構造登録者

Safari, C.,Ghosh, S.,Andersson, R.,Johannesson, J.,Donoso, A.V.,Bath, P.,Zoric, D.,Sandelin, E.,Nango, E.,Tanaka, R.,Iwata, S.,Neutze, R.,Branden, G. (登録日: 2023-07-25, 公開日: 2023-11-15, 最終更新日: 2024-03-20)

主引用文献

Safari, C.,Ghosh, S.,Andersson, R.,Johannesson, J.,Bath, P.,Uwangue, O.,Dahl, P.,Zoric, D.,Sandelin, E.,Vallejos, A.,Nango, E.,Tanaka, R.,Bosman, R.,Borjesson, P.,Dunevall, E.,Hammarin, G.,Ortolani, G.,Panman, M.,Tanaka, T.,Yamashita, A.,Arima, T.,Sugahara, M.,Suzuki, M.,Masuda, T.,Takeda, H.,Yamagiwa, R.,Oda, K.,Fukuda, M.,Tosha, T.,Naitow, H.,Owada, S.,Tono, K.,Nureki, O.,Iwata, S.,Neutze, R.,Branden, G.
Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome c oxidase.
Sci Adv, 9:eadh4179-eadh4179, 2023

PubMed Abstract: Cytochrome oxidase (CO) is part of the respiratory chain and contributes to the electrochemical membrane gradient in mitochondria as well as in many bacteria, as it uses the energy released in the reduction of oxygen to pump protons across an energy-transducing biological membrane. Here, we use time-resolved serial femtosecond crystallography to study the structural response of the active site upon flash photolysis of carbon monoxide (CO) from the reduced heme of -type CO. In contrast with the -type enzyme, our data show how CO is stabilized on Cu through interactions with a transiently ordered water molecule. These results offer a structural explanation for the extended lifetime of the Cu-CO complex in -type CO and, by extension, the extremely high oxygen affinity of the enzyme.

PubMed: 38064560
DOI: 10.1126/sciadv.adh4179

実験手法

X-RAY DIFFRACTION (2 Å)