8K5P

Cryo-EM structure of yeast Rat1-bound Pol II pre-termination transcription complex 2 (Pol II Rat1-PTTC2)

8K5P の概要

• エントリーDOI: 10.2210/pdb8k5p/pdb
• 関連するPDBエントリー: 8JCH
• EMDBエントリー: 36908
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC4, DNA (38-MER), ... (20 entities in total)
• 機能のキーワード: transcription termination, pol ii, rat1/rai1 spt5, transcription
• 由来する生物種: Saccharomyces cerevisiae S288C (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 834105.74

構造登録者

Zeng, Y.,Zhang, Y. (登録日: 2023-07-23, 公開日: 2024-01-31, 最終更新日: 2024-05-15)

主引用文献

Zeng, Y.,Zhang, H.W.,Wu, X.X.,Zhang, Y.
Structural basis of exoribonuclease-mediated mRNA transcription termination.
Nature, 628:887-893, 2024

PubMed Abstract: Efficient termination is required for robust gene transcription. Eukaryotic organisms use a conserved exoribonuclease-mediated mechanism to terminate the mRNA transcription by RNA polymerase II (Pol II). Here we report two cryogenic electron microscopy structures of Saccharomyces cerevisiae Pol II pre-termination transcription complexes bound to the 5'-to-3' exoribonuclease Rat1 and its partner Rai1. Our structures show that Rat1 displaces the elongation factor Spt5 to dock at the Pol II stalk domain. Rat1 shields the RNA exit channel of Pol II, guides the nascent RNA towards its active centre and stacks three nucleotides at the 5' terminus of the nascent RNA. The structures further show that Rat1 rotates towards Pol II as it shortens RNA. Our results provide the structural mechanism for the Rat1-mediated termination of mRNA transcription by Pol II in yeast and the exoribonuclease-mediated termination of mRNA transcription in other eukaryotes.

PubMed: 38538796
DOI: 10.1038/s41586-024-07240-3

実験手法

ELECTRON MICROSCOPY (2.8 Å)