8K57

Crystal structure of sulfur transferase from Frondihabitans sp. PAMC28461 crystallized in the I21 space group

Summary for 8K57

• Entry DOI: 10.2210/pdb8k57/pdb
• Related: 8K55
• Descriptor: sulfur transferase (2 entities in total)
• Functional Keywords: sulfur transferase, frondihabitans sp. pamc28461, detoxification, transferase
• Biological source: Frondihabitans sp.
• Total number of polymer chains: 1
• Total formula weight: 33661.04

Authors

Nguyen, D.L.,Do, H. (deposition date: 2023-07-21, release date: 2024-04-24)

Primary citation

Do, H.,Nguyen, D.L.,Ahn, Y.Y.,Nam, Y.,Kang, Y.,Oh, H.,Hwang, J.,Han, S.J.,Kim, K.,Lee, J.H.
Structural and functional characterization of sulfurtransferase from Frondihabitans sp. PAMC28461.
Plos One, 19:e0298999-e0298999, 2024

PubMed Abstract: Sulfurtransferases transfer of sulfur atoms from thiols to acceptors like cyanide. They are categorized as thiosulfate sulfurtransferases (TSTs) and 3-mercaptopyruvate sulfurtransferases (MSTs). TSTs transfer sulfur from thiosulfate to cyanide, producing thiocyanate. MSTs transfer sulfur from 3-mercaptopyruvate to cyanide, yielding pyruvate and thiocyanate. The present study aimed to isolate and characterize the sulfurtransferase FrST from Frondihabitans sp. PAMC28461 using biochemical and structural analyses. FrST exists as a dimer and can be classified as a TST rather than an MST according to sequence-based clustering and enzyme activity. Furthermore, the discovery of activity over a wide temperature range and the broad substrate specificity exhibited by FrST suggest promising prospects for its utilization in industrial applications, such as the detoxification of cyanide.

PubMed: 38526988
DOI: 10.1371/journal.pone.0298999

Experimental method

X-RAY DIFFRACTION (2.38 Å)