8K4M

Anti CRISPR protein, AcrIIA13b

8K4M の概要

• エントリーDOI: 10.2210/pdb8k4m/pdb
• 分子名称: Anti CRISPR protein (2 entities in total)
• 機能のキーワード: acriia13, acriia13b, immune system, crispr-cas system, cas9, inhibitor
• 由来する生物種: Staphylococcus haemolyticus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16333.03

構造登録者

Lee, S.Y.,Park, H.H. (登録日: 2023-07-19, 公開日: 2024-07-24, 最終更新日: 2026-02-04)

主引用文献

Lee, S.Y.,Park, H.H.
Investigating the molecular mechanisms underlying the anti-CRISPR function of AcrIIA13b protein.
Febs J., 2025

PubMed Abstract: The CRISPR-Cas systems of adaptive immunity in bacteria and archaea provide resistance against phages and other mobile genetic elements. Counteractive anti-CRISPR (Acr) proteins in phages and archaeal viruses impede these CRISPR-Cas systems. Although CRISPR-Cas systems have revolutionized genome editing, potential off-target events remain a safety concern. Hence, a thorough comprehension of the structural and molecular basis of diverse Acrs is imperative to unravel the fundamental mechanisms governing CRISPR-Cas regulation. Here, we present the structure of AcrIIA13b from Staphylococcus haemolyticus and analyze its structural and functional features to reveal the molecular basis underlying the inhibition of Cas9 by AcrIIA13b. Our structural analysis shows that AcrIIA13b eliminates the cleavage activity of Staphylococcus aureus Cas9 (SauCas9) by blocking the PAM-binding region of Cas9 so that Cas9 cannot recognize the target DNA. In addition, we demonstrate that the 15 amino acid residues at the N terminus of AcrIIA13b, which were revealed to be important for its dimerization, are critical for its inhibitory activity against Cas9. Our findings shed light on the molecular basis of AcrIIA13b-mediated CRISPR-Cas inhibition and provide valuable insights into the arms race between bacteria and phages.

PubMed: 41137484
DOI: 10.1111/febs.70304

実験手法

X-RAY DIFFRACTION (1.53 Å)