8K44
Structure of VP9 in Banna virus
Summary for 8K44
| Entry DOI | 10.2210/pdb8k44/pdb |
| Related | 8K42 |
| EMDB information | 36872 |
| Descriptor | VP9 (1 entity in total) |
| Functional Keywords | receptor binding, spike, reovirus, viral protein |
| Biological source | Banna virus |
| Total number of polymer chains | 3 |
| Total formula weight | 91743.53 |
| Authors | |
| Primary citation | Li, Z.,Xia, H.,Rao, G.,Fu, Y.,Chong, T.,Tian, K.,Yuan, Z.,Cao, S. Cryo-EM structures of Banna virus in multiple states reveal stepwise detachment of viral spikes. Nat Commun, 15:2284-2284, 2024 Cited by PubMed Abstract: Banna virus (BAV) is the prototype Seadornavirus, a class of reoviruses for which there has been little structural study. Here, we report atomic cryo-EM structures of three states of BAV virions-surrounded by 120 spikes (full virions), 60 spikes (partial virions), or no spikes (cores). BAV cores are double-layered particles similar to the cores of other non-turreted reoviruses, except for an additional protein component in the outer capsid shell, VP10. VP10 was identified to be a cementing protein that plays a pivotal role in the assembly of BAV virions by directly interacting with VP2 (inner capsid), VP8 (outer capsid), and VP4 (spike). Viral spikes (VP4/VP9 heterohexamers) are situated on top of VP10 molecules in full or partial virions. Asymmetrical electrostatic interactions between VP10 monomers and VP4 trimers are disrupted by high pH treatment, which is thus a simple way to produce BAV cores. Low pH treatment of BAV virions removes only the flexible receptor binding protein VP9 and triggers significant conformational changes in the membrane penetration protein VP4. BAV virions adopt distinct spatial organization of their surface proteins compared with other well-studied reoviruses, suggesting that BAV may have a unique mechanism of penetration of cellular endomembranes. PubMed: 38480794DOI: 10.1038/s41467-024-46624-x PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.8 Å) |
Structure validation
Download full validation report
