8K2D

Cryo-EM structure of the yeast 80S ribosome with tigecycline, eEF2, Stm1 and eIF5A

これはPDB形式変換不可エントリーです。

8K2D の概要

• エントリーDOI: 10.2210/pdb8k2d/pdb
• EMDBエントリー: 36839
• 分子名称: 18S rRNA, Small ribosomal subunit protein uS7, 40S ribosomal protein S6-A, ... (88 entities in total)
• 機能のキーワード: 80s ribosome, tigecycline, antibiotic, ribosome
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 84
• 化学式量合計: 3327244.98

構造登録者

Buschauer, R.,Beckmann, R.,Cheng, J. (登録日: 2023-07-12, 公開日: 2024-07-10, 最終更新日: 2024-10-30)

主引用文献

Li, X.,Wang, M.,Denk, T.,Buschauer, R.,Li, Y.,Beckmann, R.,Cheng, J.
Structural basis for differential inhibition of eukaryotic ribosomes by tigecycline.
Nat Commun, 15:5481-5481, 2024

PubMed Abstract: Tigecycline is widely used for treating complicated bacterial infections for which there are no effective drugs. It inhibits bacterial protein translation by blocking the ribosomal A-site. However, even though it is also cytotoxic for human cells, the molecular mechanism of its inhibition remains unclear. Here, we present cryo-EM structures of tigecycline-bound human mitochondrial 55S, 39S, cytoplasmic 80S and yeast cytoplasmic 80S ribosomes. We find that at clinically relevant concentrations, tigecycline effectively targets human 55S mitoribosomes, potentially, by hindering A-site tRNA accommodation and by blocking the peptidyl transfer center. In contrast, tigecycline does not bind to human 80S ribosomes under physiological concentrations. However, at high tigecycline concentrations, in addition to blocking the A-site, both human and yeast 80S ribosomes bind tigecycline at another conserved binding site restricting the movement of the L1 stalk. In conclusion, the observed distinct binding properties of tigecycline may guide new pathways for drug design and therapy.

PubMed: 38942792
DOI: 10.1038/s41467-024-49797-7

実験手法

ELECTRON MICROSCOPY (3.2 Å)