8K15

The Streptococcus azizii ORF-less Group IIC intron HYER2 at apo state

8K15 の概要

• エントリーDOI: 10.2210/pdb8k15/pdb
• EMDBエントリー: 36786
• 分子名称: RNA (470-MER), MAGNESIUM ION (2 entities in total)
• 機能のキーワード: orf-less group iic intron, ribozymes, rna
• 由来する生物種: Streptococcus azizii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 201165.23

構造登録者

Zhu, H.Z.,Liu, J.J.G. (登録日: 2023-07-10, 公開日: 2024-05-15)

主引用文献

Liu, Z.X.,Zhang, S.,Zhu, H.Z.,Chen, Z.H.,Yang, Y.,Li, L.Q.,Lei, Y.,Liu, Y.,Li, D.Y.,Sun, A.,Li, C.P.,Tan, S.Q.,Wang, G.L.,Shen, J.Y.,Jin, S.,Gao, C.,Liu, J.G.
Hydrolytic endonucleolytic ribozyme (HYER) is programmable for sequence-specific DNA cleavage.
Science, 383:eadh4859-eadh4859, 2024

PubMed Abstract: Ribozymes are catalytic RNAs with diverse functions including self-splicing and polymerization. This work aims to discover natural ribozymes that behave as hydrolytic and sequence-specific DNA endonucleases, which could be repurposed as DNA manipulation tools. Focused on bacterial group II-C introns, we found that many systems without intron-encoded protein propagate multiple copies in their resident genomes. These introns, named HYdrolytic Endonucleolytic Ribozymes (HYERs), cleaved RNA, single-stranded DNA, bubbled double-stranded DNA (dsDNA), and plasmids in vitro. HYER1 generated dsDNA breaks in the mammalian genome. Cryo-electron microscopy analysis revealed a homodimer structure for HYER1, where each monomer contains a Mg-dependent hydrolysis pocket and captures DNA complementary to the target recognition site (TRS). Rational designs including TRS extension, recruiting sequence insertion, and heterodimerization yielded engineered HYERs showing improved specificity and flexibility for DNA manipulation.

PubMed: 38301022
DOI: 10.1126/science.adh4859

実験手法

ELECTRON MICROSCOPY (2.58 Å)