8JXZ

Chitin binding SusD-like protein AqSusD in complex with (GlcNAc)3

8JXZ の概要

• エントリーDOI: 10.2210/pdb8jxz/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: SusD-like protein AqSusD, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: susd-like protein, sugar binding protein
• 由来する生物種: Aquimarina
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58794.59

構造登録者

Yang, J. (登録日: 2023-07-01, 公開日: 2023-11-01, 最終更新日: 2024-02-28)

主引用文献

Yang, J.,Zhang, L.,Lin, S.,Li, W.,Liu, C.,Yan, J.,Li, S.,Long, L.
Structural insights of a SusD-like protein in marine Bacteroidetes bacteria reveal the molecular basis for chitin recognition and acquisition.
Febs J., 291:584-595, 2024

PubMed Abstract: Efficient recognition and transportation of chitin oligosaccharides are crucial steps for the utilization of chitin by heterotrophic bacteria. In this study, we employed structural biological and biochemical approaches to investigate the substrate recognition and acquisition mechanism of a novel chitin-binding SusD-like protein, AqSusD, which is derived from the chitin utilization gene cluster of a marine Bacteroides strain (Aquimarina sp. SCSIO 21287). We resolved the crystal structures of the AqSusD apo-protein and its complex with chitin oligosaccharides. Our results revealed that some crucial residues (Gln67, Phe87, and Asp276) underwent significant conformational changes to form tighter substrate binding sites for ligand binding. Moreover, we identified the functions of key amino acid residues and discovered that π-π stacking and hydrogen bonding between AqSusD and the ligand played significant roles in recognition of the protein for chitin oligosaccharide binding. Based on our findings and previous investigations, we put forward a model for the mechanism of chitin oligosaccharide recognition, capture, and transport by AqSusD, in collaboration with the membrane protein AqSusC. Our study deepens the understanding of the molecular-level "selfish" use of polysaccharides such as chitin by Bacteroides.

PubMed: 37845429
DOI: 10.1111/febs.16974

実験手法

X-RAY DIFFRACTION (2.2 Å)