8JWH

Cryo-EM structure of apo-state huamn angiotensin-converting enzyme 2 (ACE2)

8JWH の概要

• エントリーDOI: 10.2210/pdb8jwh/pdb
• EMDBエントリー: 36683
• 分子名称: Processed angiotensin-converting enzyme 2 (1 entity in total)
• 機能のキーワード: receptor, sars-cov-2, viral infection, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 69153.66

構造登録者

Yang, Z.,Wang, H.W. (登録日: 2023-06-29, 公開日: 2024-07-03, 最終更新日: 2025-01-22)

主引用文献

Yang, Z.,Fan, J.,Wang, J.,Fan, X.,Ouyang, Z.,Wang, H.W.,Zhou, X.
Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects.
Nat.Methods, 21:1023-1032, 2024

PubMed Abstract: Addressing interfacial effects during specimen preparation in cryogenic electron microscopy remains challenging. Here we introduce ESI-cryoPrep, a specimen preparation method based on electrospray ionization in native mass spectrometry, designed to alleviate issues associated with protein denaturation or preferred orientation induced by macromolecule adsorption at interfaces. Through fine-tuning spraying parameters, we optimized protein integrity preservation and achieved the desired ice thickness for analyzing target macromolecules. With ESI-cryoPrep, we prepared high-quality cryo-specimens of five proteins and obtained three-dimensional reconstructions at near-atomic resolution. Our findings demonstrate that ESI-cryoPrep effectively confines macromolecules within the middle of the thin layer of amorphous ice, facilitating the preparation of blotting-free vitreous samples. The protective mechanism, characterized by the uneven distribution of charged biomolecules of varying sizes within charged droplets, prevents the adsorption of target biomolecules at air-water or graphene-water interfaces, thereby avoiding structural damage to the protein particles or the introduction of dominant orientation issues.

PubMed: 38664529
DOI: 10.1038/s41592-024-02247-0

実験手法

ELECTRON MICROSCOPY (3.34 Å)