8JWD

Histidine kinase QseE sensor domain of Escherichia coli O157:H7

8JWD の概要

• エントリーDOI: 10.2210/pdb8jwd/pdb
• 分子名称: histidine kinase, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: enterohemorrhagic escherichia coli, two-component systems, inner membrane protein, signaling protein, transferase
• 由来する生物種: Escherichia coli O157:H7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16590.34

構造登録者

Matsumoto, K.,Fukuda, Y.,Inoue, T. (登録日: 2023-06-28, 公開日: 2023-11-15, 最終更新日: 2024-10-23)

主引用文献

Matsumoto, K.,Fukuda, Y.,Inoue, T.
Crystal structures of QseE and QseG: elements of a three-component system from Escherichia coli.
Acta Crystallogr.,Sect.F, 79:285-293, 2023

PubMed Abstract: Bacteria regulate virulence by using two-component systems (TCSs) composed of a histidine kinase (HK) and a response regulator (RR). TCSs respond to environmental signals and change gene-expression levels. The HK QseE and the RR QseF regulate the virulence of Enterobacteriaceae bacteria such as enterohemorrhagic Escherichia coli. The operon encoding QseE/QseF also contains a gene encoding an outer membrane lipoprotein, qseG. The protein product QseG interacts with QseE in the periplasmic space to control the activity of QseE and constitutes a unique QseE/F/G three-component system. However, the structural bases of their functions are unknown. Here, crystal structures of the periplasmic regions of QseE and QseG were determined with the help of AlphaFold models. The periplasmic region of QseE has a helix-bundle structure as found in some HKs. The QseG structure is composed of an N-terminal globular domain and a long C-terminal helix forming a coiled-coil-like structure that contributes to dimerization. Comparison of QseG structures obtained from several crystallization conditions shows that QseG has structural polymorphisms at the C-terminus of the coiled-coil structure, indicating that the C-terminus is flexible. The C-terminal flexibility is derived from conserved hydrophilic residues that reduce the hydrophobic interaction at the coiled-coil interface. Electrostatic surface analysis suggests that the C-terminal coiled-coil region can interact with QseE. The observed structural fluctuation of the C-terminus of QseG is probably important for interaction with QseE.

PubMed: 37877621
DOI: 10.1107/S2053230X23009123

実験手法

X-RAY DIFFRACTION (1.33 Å)