Summary for 8JMV
| Entry DOI | 10.2210/pdb8jmv/pdb |
| EMDB information | 36427 |
| Descriptor | Flagella (1 entity in total) |
| Functional Keywords | flagella, motor, fibril, protein fibril |
| Biological source | Bacillus amyloliquefaciens (Bacillus velezensis) |
| Total number of polymer chains | 33 |
| Total formula weight | 1159251.72 |
| Authors | |
| Primary citation | Cheng, Y.,Han, J.,Song, M.,Zhang, S.,Cao, Q. Serine peptidase Vpr forms enzymatically active fibrils outside Bacillus bacteria revealed by cryo-EM. Nat Commun, 14:7503-7503, 2023 Cited by PubMed Abstract: Bacteria develop a variety of extracellular fibrous structures crucial for their survival, such as flagella and pili. In this study, we use cryo-EM to identify protein fibrils surrounding lab-cultured Bacillus amyloiquefaciens and discover an unreported fibril species in addition to the flagellar fibrils. These previously unknown fibrils are composed of Vpr, an extracellular serine peptidase. We find that Vpr assembles into fibrils in an enzymatically active form, potentially representing a strategy of enriching Vpr activities around bacterial cells. Vpr fibrils are also observed under other culture conditions and around other Bacillus bacteria, such as Bacillus subtilis, which may suggest a general mechanism across all Bacillus bacterial groups. Taken together, our study reveals fibrils outside the bacterial cell and sheds light on the physiological role of these extracellular fibrils. PubMed: 37980359DOI: 10.1038/s41467-023-43359-z PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.9 Å) |
Structure validation
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