8JIC
human telomere two-quartet G-quadruplex at pH 7.0
Summary for 8JIC
| Entry DOI | 10.2210/pdb8jic/pdb |
| Descriptor | DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*G)-3') (1 entity in total) |
| Functional Keywords | human telomere repeat, topology, dna |
| Biological source | Homo sapiens |
| Total number of polymer chains | 1 |
| Total formula weight | 6958.48 |
| Authors | Wang, B.,Galer, P.,Plavec, J. (deposition date: 2023-05-26, release date: 2024-04-03, Last modification date: 2024-05-08) |
| Primary citation | Galer, P.,Wang, B.,Plavec, J.,Sket, P. Unveiling the structural mechanism of a G-quadruplex pH-Driven switch. Biochimie, 214:73-82, 2023 Cited by PubMed Abstract: The human telomere oligonucleotide, d[TAGGG(TTAGGG)TTAGG] (TAGGG), can adopt two distinct 2-G-quartet G-quadruplex structures at pH 7.0 and 5.0, referred to as the TD and KDH forms, respectively. By using a combination of NMR and computational techniques, we determined high-resolution structures of both forms, which revealed unique loop architectures, base triples, and base pairs that play a crucial role in the pH-driven structural transformation of TAGGG. Our study demonstrated that TAGGG represents a reversible pH-driven switch system where the stability and pH-induced structural transformation of the G-quadruplexes are influenced by the terminal residues and base triples. Gaining insight into the factors that regulate the formation of G-quadruplexes and their pH-sensitive structural equilibrium holds great potential for the rational design of novel DNA based pH-driven switches. These advancements in understanding create exciting opportunities for applications in the field of nanotechnology, specifically in the development of bio-nano-motors. PubMed: 37573019DOI: 10.1016/j.biochi.2023.08.002 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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