8JFV

Crystal structure of Catabolite repressor acivator from E. coli in complex with sulisobenzone

8JFV の概要

• エントリーDOI: 10.2210/pdb8jfv/pdb
• 分子名称: Catabolite repressor/activator, Sulisobenzone, PHOSPHATE ION, ... (6 entities in total)
• 機能のキーワード: transcription factor, bacterial, sulsiobenzone, transcription
• 由来する生物種: Escherichia coli 536
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76806.31

構造登録者

Neetu, N.,Sharma, M.,Mahto, J.K.,Kumar, P. (登録日: 2023-05-19, 公開日: 2024-04-24, 最終更新日: 2024-11-20)

主引用文献

Neetu, N.,Mahto, J.K.,Sharma, M.,Katiki, M.,Dhaka, P.,Roy, P.,Tomar, S.,Narayan, A.,Yernool, D.,Kumar, P.
Sulisobenzone is a potent inhibitor of the global transcription factor Cra.
J.Struct.Biol., 215:108034-108034, 2023

PubMed Abstract: Transcription is carried out by the RNA polymerase and is regulated through a series of interactions with transcription factors. Catabolite activator repressor (Cra), a LacI family transcription factor regulates the virulence gene expression in Enterohaemorrhagic Escherichia coli (EHEC) and thus is a promising drug target for the discovery of antivirulence molecules. Here, we report the crystal structure of the effector molecule binding domain of Cra from E. coli (EcCra) in complex with HEPES molecule. Based on the EcCra-HEPES complex structure, ligand screening was performed that identified sulisobenzone as an potential inhibitor of EcCra. The electrophoretic mobility shift assay (EMSA) and in vitro transcription assay validated the sulisobenzone binding to EcCra. Moreover, the isothermal titration calorimetry (ITC) experiments demonstrated a 40-fold higher binding affinity of sulisobenzone (K 360 nM) compared to the HEPES molecule. Finally, the sulisobenzone bound EcCra complex crystal structure was determined to elucidate the binding mechanism of sulisobenzone to the effector binding pocket of EcCra. Together, this study suggests that sulisobenzone may be a promising candidate that can be studied and developed as an effective antivirulence agent against EHEC.

PubMed: 37805153
DOI: 10.1016/j.jsb.2023.108034

実験手法

X-RAY DIFFRACTION (3.05 Å)