8JDL

Structure of the Human cytoplasmic Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAU) (non-rotated state)

これはPDB形式変換不可エントリーです。

8JDL の概要

• エントリーDOI: 10.2210/pdb8jdl/pdb
• 関連するPDBエントリー: 7Y7C 7Y7D 7Y7E 7Y7F 7Y7G 7Y7H
• EMDBエントリー: 36180
• 分子名称: mRNA, 60S ribosomal protein L6, 60S ribosomal protein L7, ... (82 entities in total)
• 機能のキーワード: trna modifications, decoding, ribosome
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 79
• 化学式量合計: 3791567.77

構造登録者

Ishiguro, K.,Yokoyama, T.,Shirouzu, M.,Suzuki, T. (登録日: 2023-05-14, 公開日: 2023-12-06, 最終更新日: 2025-02-12)

主引用文献

Zhao, X.,Ma, D.,Ishiguro, K.,Saito, H.,Akichika, S.,Matsuzawa, I.,Mito, M.,Irie, T.,Ishibashi, K.,Wakabayashi, K.,Sakaguchi, Y.,Yokoyama, T.,Mishima, Y.,Shirouzu, M.,Iwasaki, S.,Suzuki, T.,Suzuki, T.
Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth.
Cell, 186:5517-, 2023

PubMed Abstract: Transfer RNA (tRNA) modifications are critical for protein synthesis. Queuosine (Q), a 7-deaza-guanosine derivative, is present in tRNA anticodons. In vertebrate tRNAs for Tyr and Asp, Q is further glycosylated with galactose and mannose to generate galQ and manQ, respectively. However, biogenesis and physiological relevance of Q-glycosylation remain poorly understood. Here, we biochemically identified two RNA glycosylases, QTGAL and QTMAN, and successfully reconstituted Q-glycosylation of tRNAs using nucleotide diphosphate sugars. Ribosome profiling of knockout cells revealed that Q-glycosylation slowed down elongation at cognate codons, UAC and GAC (GAU), respectively. We also found that galactosylation of Q suppresses stop codon readthrough. Moreover, protein aggregates increased in cells lacking Q-glycosylation, indicating that Q-glycosylation contributes to proteostasis. Cryo-EM of human ribosome-tRNA complex revealed the molecular basis of codon recognition regulated by Q-glycosylations. Furthermore, zebrafish qtgal and qtman knockout lines displayed shortened body length, implying that Q-glycosylation is required for post-embryonic growth in vertebrates.

PubMed: 37992713
DOI: 10.1016/j.cell.2023.10.026

実験手法

ELECTRON MICROSCOPY (2.42 Å)