8JCC

Human histone H2B variant H2BFWT Cryo-EM structure with 601 DNA sequence

8JCC の概要

• エントリーDOI: 10.2210/pdb8jcc/pdb
• EMDBエントリー: 36157
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (6 entities in total)
• 機能のキーワード: nucleosome, histone variant, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 205854.39

構造登録者

Ding, D.B.,Ishibashi, T. (登録日: 2023-05-10, 公開日: 2024-05-15, 最終更新日: 2025-07-02)

主引用文献

Ding, D.,Pang, M.Y.H.,Deng, M.,Nguyen, T.T.,Liu, Y.,Sun, X.,Xu, Z.,Zhang, Y.,Zhai, Y.,Yan, Y.,Ishibashi, T.
Testis-specific H2B.W1 disrupts nucleosome integrity by reducing DNA-histone interactions.
Nucleic Acids Res., 52:11612-11625, 2024

PubMed Abstract: Multiple testis-specific histone variants are involved in the dynamic chromatin transitions during spermatogenesis. H2B.W1 (previously called H2BFWT) is an H2B variant specific to primate testis with hitherto unclear functions, although its single-nucleotide polymorphisms (SNPs) are closely associated with male non-obstructive infertility. Here, we found that H2B.W1 is only expressed in the mid-late spermatogonia stages, and H2B.W1 nucleosomes are defined by a more flexible structure originating from weakened interactions between histones and DNA. Furthermore, one of its SNPs, H2B.W1-H100R, which is associated with infertility, further destabilizes the nucleosomes and increases the nucleosome unwrapping rate by interfering with the R100 and H4 K91/R92 interaction. Our results suggest that destabilizing H2B.W1 containing nucleosomes might change the chromatin structure of spermatogonia, and that H2B.W1-H100R enhances the nucleosome-destabilizing effects, leading to infertility.

PubMed: 39329259
DOI: 10.1093/nar/gkae825

実験手法

ELECTRON MICROSCOPY (3.42 Å)