8J9I

Cryo-EM structure of Euglena gracilis complex I, turnover state

これはPDB形式変換不可エントリーです。

8J9I の概要

• エントリーDOI: 10.2210/pdb8j9i/pdb
• EMDBエントリー: 36108
• 分子名称: NDUFS1A, NDUFA7, NDUFA8, ... (71 entities in total)
• 機能のキーワード: electron transport chain, respiratory complex, membrane protein, euglena gracilis, electron transport
• 由来する生物種: Euglena gracilis; 詳細
• タンパク質・核酸の鎖数: 61
• 化学式量合計: 1793987.28

構造登録者

Wu, M.C.,He, Z.X.,Tian, H.T.,Hu, Y.Q.,Han, F.Z.,Zhou, L. (登録日: 2023-05-03, 公開日: 2024-02-28, 最終更新日: 2024-10-09)

主引用文献

He, Z.,Wu, M.,Tian, H.,Wang, L.,Hu, Y.,Han, F.,Zhou, J.,Wang, Y.,Zhou, L.
Euglena's atypical respiratory chain adapts to the discoidal cristae and flexible metabolism.
Nat Commun, 15:1628-1628, 2024

PubMed Abstract: Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron transport chain. While structures of the electron transport chain complexes and supercomplexes of most other eukaryotic clades have been reported, no similar structure is currently available for Discoba, limiting the understandings of its core metabolism and leaving a gap in the evolutionary tree of eukaryotic bioenergetics. Here, we report high-resolution cryo-EM structures of Euglena's respirasome I + III + IV and supercomplex III + IV. A previously unreported fatty acid synthesis domain locates on the tip of complex I's peripheral arm, providing a clear picture of its atypical subunit composition identified previously. Individual complexes are re-arranged in the respirasome to adapt to the non-uniform membrane curvature of the discoidal cristae. Furthermore, Euglena's conformationally rigid complex I is deactivated by restricting ubiquinone's access to its substrate tunnel. Our findings provide structural insights for therapeutic developments against euglenozoan parasite infections.

PubMed: 38388527
DOI: 10.1038/s41467-024-46018-z

実験手法

ELECTRON MICROSCOPY (2.87 Å)