8J56

Crystal structure of the FlhDC complex from Cupriavidus necator

8J56 の概要

• エントリーDOI: 10.2210/pdb8j56/pdb
• 分子名称: Flagellar transcriptional regulator FlhD, Flagellar transcriptional regulator FlhC, ZINC ION (3 entities in total)
• 機能のキーワード: flhdc, flagellar master regulator, transcription factor, cupriavidus necator, transcription
• 由来する生物種: Cupriavidus necator; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 94421.65

構造登録者

Cho, S.Y.,Oh, H.B.,Yoon, S.I. (登録日: 2023-04-21, 公開日: 2024-02-28)

主引用文献

Cho, S.Y.,Oh, H.B.,Yoon, S.I.
Hexameric structure of the flagellar master regulator FlhDC from Cupriavidus necator and its interaction with flagellar promoter DNA.
Biochem.Biophys.Res.Commun., 672:97-102, 2023

PubMed Abstract: Bacterial flagella are assembled with ∼30 different proteins in a defined order via diverse regulatory systems. In gram-negative bacteria from the Gammaproteobacteria and Betaproteobacteria classes, the transcription of flagellar genes is strictly controlled by the master regulator FlhDC. In Gammaproteobacteria species, the FlhDC complex has been shown to activate flagellar expression by directly interacting with the promoter region in flagellar genes. To obtain the DNA-binding mechanism of FlhDC and determine the conserved and distinct structural features of Betaproteobacteria and Gammaproteobacteria FlhDCs that are necessary for their functions, we determined the crystal structure of Betaproteobacteria Cupriavidus necator FlhDC (cnFlhDC) and biochemically analyzed its DNA-binding capacity. cnFlhDC specifically recognized the promoter DNA of the class II flagellar genes flgB and flhB. cnFlhDC adopts a ring-like heterohexameric structure (cnFlhDC) and harbors two Zn-Cys clusters, as observed for Gammaproteobacteria Escherichia coli FlhDC (ecFlhDC). The cnFlhDC structure exhibits positively charged surfaces across two FlhDC subunits as a putative DNA-binding site. Noticeably, the positive patch of cnFlhDC is continuous, in contrast to the separated patches of ecFlhDC. Moreover, the ternary intersection of cnFlhDC behind the Zn-Cys cluster forms a unique protruding neutral structure, which is replaced with a charged cavity in the ecFlhDC structure.

PubMed: 37343320
DOI: 10.1016/j.bbrc.2023.06.033

実験手法

X-RAY DIFFRACTION (3.5 Å)