8J4T
GajA-GajB complex
Summary for 8J4T
| Entry DOI | 10.2210/pdb8j4t/pdb |
| EMDB information | 35977 |
| Descriptor | Endonuclease GajA, Gabija protein GajB (2 entities in total) |
| Functional Keywords | complex, dna-binding, immunity system, dna binding protein |
| Biological source | Bacillus cereus VD045 More |
| Total number of polymer chains | 8 |
| Total formula weight | 505663.81 |
| Authors | |
| Primary citation | Huo, Y.,Kong, L.,Zhang, Y.,Xiao, M.,Du, K.,Xu, S.,Yan, X.,Ma, J.,Wei, T. Structural and biochemical insights into the mechanism of the Gabija bacterial immunity system. Nat Commun, 15:836-836, 2024 Cited by PubMed Abstract: The Gabija system is a newly discovered bacterial immune system that consists of GajA and GajB. Here we report the cryo-EM structure of the Gabija complex from Bacillus cereus VD045 at 3.6 Å, which provides the direct evidence of interactions between GajA and GajB. The Gabija complex is an octameric ring structure with four GajA and four GajB. GajA is an OLD nucleases family protein, while GajB belongs to the SF1 helicases. The Gabija complex has sequence-specific DNA nuclease activity and prefers circular rather than linear DNA as substrate, its activity is more sensitive to concentrations change of nucleotides compared to GajA alone. Our data suggest a mechanism of Gabija immunity: the nuclease activity of Gabija complex is inhibited under physiological conditions, while it is activated by depletion of NTP and dNTP upon the replication and transcription of invading phages and cleave the circular DNA to prevent phage DNA replication. PubMed: 38282040DOI: 10.1038/s41467-024-45173-7 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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