8J4T

GajA-GajB complex

8J4T の概要

• エントリーDOI: 10.2210/pdb8j4t/pdb
• EMDBエントリー: 35977
• 分子名称: Endonuclease GajA, Gabija protein GajB (2 entities in total)
• 機能のキーワード: complex, dna-binding, immunity system, dna binding protein
• 由来する生物種: Bacillus cereus VD045; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 505663.81

構造登録者

Wei, T.,Ma, J.,Huo, Y. (登録日: 2023-04-20, 公開日: 2024-02-21)

主引用文献

Huo, Y.,Kong, L.,Zhang, Y.,Xiao, M.,Du, K.,Xu, S.,Yan, X.,Ma, J.,Wei, T.
Structural and biochemical insights into the mechanism of the Gabija bacterial immunity system.
Nat Commun, 15:836-836, 2024

PubMed Abstract: The Gabija system is a newly discovered bacterial immune system that consists of GajA and GajB. Here we report the cryo-EM structure of the Gabija complex from Bacillus cereus VD045 at 3.6 Å, which provides the direct evidence of interactions between GajA and GajB. The Gabija complex is an octameric ring structure with four GajA and four GajB. GajA is an OLD nucleases family protein, while GajB belongs to the SF1 helicases. The Gabija complex has sequence-specific DNA nuclease activity and prefers circular rather than linear DNA as substrate, its activity is more sensitive to concentrations change of nucleotides compared to GajA alone. Our data suggest a mechanism of Gabija immunity: the nuclease activity of Gabija complex is inhibited under physiological conditions, while it is activated by depletion of NTP and dNTP upon the replication and transcription of invading phages and cleave the circular DNA to prevent phage DNA replication.

PubMed: 38282040
DOI: 10.1038/s41467-024-45173-7

実験手法

ELECTRON MICROSCOPY (3.6 Å)