8J4C

YeeE(TsuA)-YeeD(TsuB) complex for thiosulfate uptake

8J4C の概要

• エントリーDOI: 10.2210/pdb8j4c/pdb
• 分子名称: Spirochaeta thermophila YeeE(TsuA)-YeeD(TsuB),UPF0033 domain-containing protein, SirA-like domain-containing protein (chimera), THIOSULFATE, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate (3 entities in total)
• 機能のキーワード: thiosulfate, bacterial sulfur assimilation, membrane protein, transport protein
• 由来する生物種: Spirochaeta thermophila DSM 6578; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 106666.89

構造登録者

Ikei, M.,Miyazaki, R.,Monden, K.,Naito, Y.,Takeuchi, A.,Takahashi, Y.S.,Tanaka, Y.,Ichikawa, M.,Tsukazaki, T. (登録日: 2023-04-19, 公開日: 2024-03-27, 最終更新日: 2024-10-09)

主引用文献

Ikei, M.,Miyazaki, R.,Monden, K.,Naito, Y.,Takeuchi, A.,Takahashi, Y.S.,Tanaka, Y.,Murata, K.,Mori, T.,Ichikawa, M.,Tsukazaki, T.
YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition.
Plos Biol., 22:e3002601-e3002601, 2024

PubMed Abstract: Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving a membrane protein YeeE (TsuA) in Escherichia coli was characterized. YeeE-like proteins are conserved in some bacteria, archaea, and eukaryotes. However, the precise function of YeeE, along with its potential partner protein in the thiosulfate ion uptake pathway, remained unclear. Here, we assessed selective thiosulfate transport via Spirochaeta thermophila YeeE in vitro and characterized E. coli YeeD (TsuB) as an adjacent and essential protein for YeeE-mediated thiosulfate uptake in vivo. We further showed that S. thermophila YeeD possesses thiosulfate decomposition activity and that a conserved cysteine in YeeD was modified to several forms in the presence of thiosulfate. Finally, the crystal structures of S. thermophila YeeE-YeeD fusion proteins at 3.34-Å and 2.60-Å resolutions revealed their interactions. The association was evaluated by a binding assay using purified S. thermophila YeeE and YeeD. Based on these results, a model of the sophisticated uptake of thiosulfate ions by YeeE and YeeD is proposed.

PubMed: 38656967
DOI: 10.1371/journal.pbio.3002601

実験手法

X-RAY DIFFRACTION (3.34 Å)