8IWO

The rice Na+/H+ antiporter SOS1 in an auto-inhibited state

8IWO の概要

• エントリーDOI: 10.2210/pdb8iwo/pdb
• EMDBエントリー: 35775
• 分子名称: OsSOS1, (2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl tetradecanoate (2 entities in total)
• 機能のキーワード: na+/h+ antiporter, salt stress, cation:proton antiporter family 1, membrane protein
• 由来する生物種: Oryza sativa Japonica Group (Japanese rice)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 257400.71

構造登録者

Zhang, X.Y.,Tang, L.H.,Zhang, C.R.,Nie, J.W. (登録日: 2023-03-30, 公開日: 2023-11-22, 最終更新日: 2023-11-29)

主引用文献

Zhang, X.Y.,Tang, L.H.,Nie, J.W.,Zhang, C.R.,Han, X.,Li, Q.Y.,Qin, L.,Wang, M.H.,Huang, X.,Yu, F.,Su, M.,Wang, Y.,Xu, R.M.,Guo, Y.,Xie, Q.,Chen, Y.H.
Structure and activation mechanism of the rice Salt Overly Sensitive 1 (SOS1) Na + /H + antiporter.
Nat.Plants, 9:1924-1936, 2023

PubMed Abstract: Salinity is one of the most severe abiotic stresses that adversely affect plant growth and agricultural productivity. The plant Na/H antiporter Salt Overly Sensitive 1 (SOS1) located in the plasma membrane extrudes excess Na out of cells in response to salt stress and confers salt tolerance. However, the molecular mechanism underlying SOS1 activation remains largely elusive. Here we elucidate two cryo-electron microscopy structures of rice (Oryza sativa) SOS1, a full-length protein in an auto-inhibited state and a truncated version in an active state. The SOS1 forms a dimeric architecture, with an NhaA-folded transmembrane domain portion in the membrane and an elongated cytosolic portion of multiple regulatory domains in the cytoplasm. The structural comparison shows that SOS1 adopts an elevator transport mechanism accompanied by a conformational transition of the highly conserved Pro in the unwound transmembrane helix 5 (TM), switching from an occluded conformation in the auto-inhibited state to a conducting conformation in the active state. These findings allow us to propose an inhibition-release mechanism for SOS1 activation and elucidate how SOS1 controls Na homeostasis in response to salt stress.

PubMed: 37884653
DOI: 10.1038/s41477-023-01551-5

実験手法

ELECTRON MICROSCOPY (3.09 Å)